1d9c

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{{STRUCTURE_1d9c| PDB=1d9c | SCENE= }}
{{STRUCTURE_1d9c| PDB=1d9c | SCENE= }}
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'''BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS'''
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===BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS===
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==Overview==
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The structure of bovine interferon-gamma (IFN-gamma) was determined by multiple isomorphous replacement at 2.0 A resolution. Bovine IFN-gamma crystallizes in two related crystal forms. Crystal form 1 diffracts to 2.9 A resolution and is reproducible and stable to derivatization. Crystal form 2 diffracts to 2.0 A resolution, but shows significant non-isomorphism from crystal to crystal. The previously determined structures of several different species of INF-gamma were either at too low a resolution [human, 1hig; Ealick et al. (1991), Science, 252, 698-702] or were too inaccurate [bovine, 1rfb; Samudzi &amp; Rubin (1993), Acta Cryst. D49(6), 505-512; rabbit, 2rig; Samudzi et al. (1991), J. Biol. Chem. 266(32), 21791-21797] for the structure to be solved by molecular replacement. The structure was solved in crystal form 1 using two derivatives produced by chemically modifying two free cysteine residues that were introduced by site-directed mutagenesis (Ser30Cys, Asn59Cys). After model building and refinement, the final R value was 21.8% (R(free) = 30.9%) for all data in the resolution range 8.0-2.9 A. The crystal form 1 structure was then used as a molecular-replacement model for crystal form 2 data collected from a flash-cooled crystal. Subsequent model building and refinement, using all data in the resolution range 15.0-2.0 A, gave an R value of 19.7% and an R(free) of 27.5%. Pairwise comparison of C(alpha) positions of bovine IFN-gamma (BOV) and the previously determined 1rfb and 2rig structures indicated some significant differences in the models (r.m.s.d. values for BOV to 1rfb, 4.3 A; BOV to 2rig, 4.0 A). An assessment of the quality of the structures was made using the 3D-1D algorithm [Eisenberg et al. (1992), Faraday Discuss. 93, 25-34]. The resulting statistical scoring indicated that BOV was consistent with expected criteria for a 2.0 A structure, whereas both 1rfb and 2rig fell below acceptable criteria.
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{{ABSTRACT_PUBMED_10666622}}
==About this Structure==
==About this Structure==
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[[Category: Randal, M.]]
[[Category: Randal, M.]]
[[Category: Helical homodimer]]
[[Category: Helical homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:42:02 2008''

Revision as of 19:42, 30 June 2008

Image:1d9c.png

Template:STRUCTURE 1d9c

BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS

Template:ABSTRACT PUBMED 10666622

About this Structure

1D9C is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species., Randal M, Kossiakoff AA, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):14-24. PMID:10666622

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