1eyo
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(New page: 200px<br /><applet load="1eyo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyo" /> '''SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM C...)
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Revision as of 12:19, 20 November 2007
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SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA
Overview
The three-dimensional solution structure of conotoxin TVIIA, a 30-residue, polypeptide from the venom of the piscivorous cone snail Conus tulipa, has, been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine, residues which form a 'four-loop' structural framework common to many, peptides from Conus venoms including the omega-, delta-, kappa-, and, muO-conotoxins. However, TVIIA does not belong to these well-characterized, pharmacological classes of conotoxins, but displays high sequence identity, with conotoxin GS, a muscle sodium channel blocker from Conus geographus., Structure calculations were based on 562 interproton distance restraints, inferred from NOE data, together with 18 backbone and nine side-chain, torsion angle restraints derived from spin-spin coupling constants. The, final family of 20 structures had mean pairwise rms differences over, residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A, for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and, 23-27) and several beta turns. The core of the molecule is formed by three, disulfide bonds which form a cystine knot motif common to many toxic and, inhibitory polypeptides. The global fold, molecular shape and distribution, of amino-acid sidechains in TVIIA is similar to that previously reported, for conotoxin GS, and comparison with other four-loop conotoxin structures, provides further indication that TVIIA and GS represent a new and distinct, subgroup of this structural family. The structure of TVIIA determined in, this study provides the basis for determining a structure-activity, relationship for these molecules and their interaction with target, receptors.
About this Structure
1EYO is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure., Hill JM, Alewood PF, Craik DJ, Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497
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