This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1eyo
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1eyo" size="450" color="white" frame="true" align="right" spinBox="true" caption="1eyo" /> '''SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM C...)
Next diff →
Revision as of 12:19, 20 November 2007
|
SOLUTION STRUCTURE OF CONOTOXIN TVIIA FROM CONUS TULIPA
Overview
The three-dimensional solution structure of conotoxin TVIIA, a 30-residue, polypeptide from the venom of the piscivorous cone snail Conus tulipa, has, been determined using 2D 1H NMR spectroscopy. TVIIA contains six cysteine, residues which form a 'four-loop' structural framework common to many, peptides from Conus venoms including the omega-, delta-, kappa-, and, muO-conotoxins. However, TVIIA does not belong to these well-characterized, pharmacological classes of conotoxins, but displays high sequence identity, with conotoxin GS, a muscle sodium channel blocker from Conus geographus., Structure calculations were based on 562 interproton distance restraints, inferred from NOE data, together with 18 backbone and nine side-chain, torsion angle restraints derived from spin-spin coupling constants. The, final family of 20 structures had mean pairwise rms differences over, residues 2-27 of 0.18+/-0.05 A for the backbone atoms and 1.39+/-0.33 A, for all heavy atoms. The structure consists of a triple-stranded, antiparallel beta sheet with +2x, -1 topology (residues 7-9, 16-20 and, 23-27) and several beta turns. The core of the molecule is formed by three, disulfide bonds which form a cystine knot motif common to many toxic and, inhibitory polypeptides. The global fold, molecular shape and distribution, of amino-acid sidechains in TVIIA is similar to that previously reported, for conotoxin GS, and comparison with other four-loop conotoxin structures, provides further indication that TVIIA and GS represent a new and distinct, subgroup of this structural family. The structure of TVIIA determined in, this study provides the basis for determining a structure-activity, relationship for these molecules and their interaction with target, receptors.
About this Structure
1EYO is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa 2. Three-dimensional solution structure., Hill JM, Alewood PF, Craik DJ, Eur J Biochem. 2000 Aug;267(15):4649-57. PMID:10903497
Page seeded by OCA on Tue Nov 20 14:26:20 2007
