1dc9

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{{STRUCTURE_1dc9| PDB=1dc9 | SCENE= }}
{{STRUCTURE_1dc9| PDB=1dc9 | SCENE= }}
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'''PROPERTIES AND CRYSTAL STRUCTURE OF A BETA-BARREL FOLDING MUTANT, V60N INTESTINAL FATTY ACID BINDING PROTEIN (IFABP)'''
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===PROPERTIES AND CRYSTAL STRUCTURE OF A BETA-BARREL FOLDING MUTANT, V60N INTESTINAL FATTY ACID BINDING PROTEIN (IFABP)===
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==Overview==
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A mutant of a beta-barrel protein, rat intestinal fatty acid binding protein, was predicted to be more stable than the wild-type protein due to a novel hydrogen bond. Equilibrium denaturation studies indicated the opposite: the V60N mutant protein was less stable. The folding transitions followed by CD and fluorescence were reversible and two-state for both mutant and wild-type protein. However, the rates of denaturation and renaturation of V60N were faster. During unfolding, the initial rate was associated with 75-80% of the fluorescence and all of the CD amplitude change. A subsequent rate accounted for the remaining fluorescence change for both proteins; thus the intermediate state lacked secondary structure. During folding, one rate was detected by both fluorescence and CD after an initial burst phase for both wild-type and mutant. An additional slower folding rate was detected by fluorescence for the mutant protein. The structure of the V60N mutant has been obtained and is nearly identical to prior crystal structures of IFABP. Analysis of mean differences in hydrogen bond and van der Waals interactions did not readily account for the stability loss due to the mutation. However, significant average differences of the solvent accessible surface and crystallographic displacement factors suggest entropic destabilization.
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(as it appears on PubMed at http://www.pubmed.gov), where 10692339 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10692339}}
==About this Structure==
==About this Structure==
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[[Category: Intracellular lipid binding protein]]
[[Category: Intracellular lipid binding protein]]
[[Category: Mutant]]
[[Category: Mutant]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 22:48:41 2008''

Revision as of 19:48, 30 June 2008

Image:1dc9.png

Template:STRUCTURE 1dc9

PROPERTIES AND CRYSTAL STRUCTURE OF A BETA-BARREL FOLDING MUTANT, V60N INTESTINAL FATTY ACID BINDING PROTEIN (IFABP)

Template:ABSTRACT PUBMED 10692339

About this Structure

1DC9 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Properties and crystal structure of a beta-barrel folding mutant., Ropson IJ, Yowler BC, Dalessio PM, Banaszak L, Thompson J, Biophys J. 2000 Mar;78(3):1551-60. PMID:10692339

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