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| - | [[Image:1dhs.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1dhs| PDB=1dhs | SCENE= }} | | {{STRUCTURE_1dhs| PDB=1dhs | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE NAD COMPLEX OF HUMAN DEOXYHYPUSINE SYNTHASE'''
| + | ===CRYSTAL STRUCTURE OF THE NAD COMPLEX OF HUMAN DEOXYHYPUSINE SYNTHASE=== |
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| - | ==Overview==
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| - | BACKGROUND: Eukaryotic initiation factor 5A (elF-5A) contains an unusual amino acid, hypusine [N epsilon-(4-aminobutyl-2-hydroxy)lysine]. The first step in the post-translational formation of hypusine is catalysed by the enzyme deoxyhypusine synthase (DHS). The modified version of elF-5A, and DHS, are required for eukaryotic cell proliferation. Knowledge of the three-dimensional structure of this key enzyme should permit the design of specific inhibitors that may be useful as anti-proliferative agents. RESULTS: The crystal structure of human DHS with bound NAD cofactor has been determined and refined at 2.2 A resolution. The enzyme is a tetramer of four identical subunits arranged with 222 symmetry; each subunit contains a nucleotide-binding (or Rossmann) fold. The tetramer comprises two tightly associated dimers and contains four active sites, two in each dimer interface. The catalytic portion of each active site is located in one subunit while the NAD-binding site is located in the other. The entrance to the active-site cavity is blocked by a two-turn alpha helix, part of a third subunit, to which it is joined by an extended loop. CONCLUSIONS: The active site of DHS is a cavity buried below the surface of the enzyme at the interface between two subunits. In the conformation observed here, the substrate-binding site is inaccessible and we propose that the reaction steps carried out by the enzyme must be accompanied by significant conformational changes, the least of which would be the displacement of the two-turn alpha helix.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_9493264}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9493264 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9493264}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Structure]] | | [[Category: Structure]] |
| | [[Category: Subunit interaction]] | | [[Category: Subunit interaction]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:51:36 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:04:53 2008'' |
Revision as of 20:04, 30 June 2008
Template:STRUCTURE 1dhs
CRYSTAL STRUCTURE OF THE NAD COMPLEX OF HUMAN DEOXYHYPUSINE SYNTHASE
Template:ABSTRACT PUBMED 9493264
About this Structure
1DHS is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the NAD complex of human deoxyhypusine synthase: an enzyme with a ball-and-chain mechanism for blocking the active site., Liao DI, Wolff EC, Park MH, Davies DR, Structure. 1998 Jan 15;6(1):23-32. PMID:9493264
Page seeded by OCA on Mon Jun 30 23:04:53 2008
