1dmu

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{{STRUCTURE_1dmu| PDB=1dmu | SCENE= }}
{{STRUCTURE_1dmu| PDB=1dmu | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE RESTRICTION ENDONUCLEASE BGLI (E.C.3.1.21.4) BOUND TO ITS DNA RECOGNITION SEQUENCE'''
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===CRYSTAL STRUCTURE OF THE RESTRICTION ENDONUCLEASE BGLI (E.C.3.1.21.4) BOUND TO ITS DNA RECOGNITION SEQUENCE===
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==Overview==
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The crystal structure of the type II restriction endonuclease BglI bound to DNA containing its specific recognition sequence has been determined at 2.2 A resolution. This is the first structure of a restriction endonuclease that recognizes and cleaves an interrupted DNA sequence, producing 3' overhanging ends. BglI is a homodimer that binds its specific DNA sequence with the minor groove facing the protein. Parts of the enzyme reach into both the major and minor grooves to contact the edges of the bases within the recognition half-sites. The arrangement of active site residues is strikingly similar to other restriction endonucleases, but the co-ordination of two calcium ions at the active site gives new insight into the catalytic mechanism. Surprisingly, the core of a BglI subunit displays a striking similarity to subunits of EcoRV and PvuII, but the dimer structure is dramatically different. The BglI-DNA complex demonstrates, for the first time, that a conserved subunit fold can dimerize in more than one way, resulting in different DNA cleavage patterns.
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(as it appears on PubMed at http://www.pubmed.gov), where 9736624 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9736624}}
==About this Structure==
==About this Structure==
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[[Category: Alpha/beta structure]]
[[Category: Alpha/beta structure]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:02:13 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:18:48 2008''

Revision as of 20:18, 30 June 2008

Image:1dmu.png

Template:STRUCTURE 1dmu

CRYSTAL STRUCTURE OF THE RESTRICTION ENDONUCLEASE BGLI (E.C.3.1.21.4) BOUND TO ITS DNA RECOGNITION SEQUENCE

Template:ABSTRACT PUBMED 9736624

About this Structure

1DMU is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of restriction endonuclease BglI bound to its interrupted DNA recognition sequence., Newman M, Lunnen K, Wilson G, Greci J, Schildkraut I, Phillips SE, EMBO J. 1998 Sep 15;17(18):5466-76. PMID:9736624

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