1dt0

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{{STRUCTURE_1dt0| PDB=1dt0 | SCENE= }}
{{STRUCTURE_1dt0| PDB=1dt0 | SCENE= }}
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'''CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS'''
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===CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS===
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==Overview==
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The gene encoding the iron-dependent superoxide dismutase from Pseudomonas ovalis was cloned from a genomic library and sequenced. The ORF differs from the previously published protein sequence, which was used for the original structure determination, at 16 positions. The differences include three additional inserted residues, one deleted residue and 12 point substitutions. The gene was subcloned and the recombinant protein overexpressed, purified and crystallized in a trigonal space group. The structure was determined by molecular replacement and was refined to 2.1 A resolution.
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(as it appears on PubMed at http://www.pubmed.gov), where 11053832 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11053832}}
==About this Structure==
==About this Structure==
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[[Category: Pseudomonas ovali]]
[[Category: Pseudomonas ovali]]
[[Category: Superoxide dismutase]]
[[Category: Superoxide dismutase]]
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Revision as of 20:34, 30 June 2008

Template:STRUCTURE 1dt0

CLONING, SEQUENCE, AND CRYSTALLOGRAPHIC STRUCTURE OF RECOMBINANT IRON SUPEROXIDE DISMUTASE FROM PSEUDOMONAS OVALIS

Template:ABSTRACT PUBMED 11053832

About this Structure

1DT0 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

Cloning, sequence and crystallographic structure of recombinant iron superoxide dismutase from Pseudomonas ovalis., Bond CJ, Huang J, Hajduk R, Flick KE, Heath PJ, Stoddard BL, Acta Crystallogr D Biol Crystallogr. 2000 Nov;56(Pt 11):1359-66. PMID:11053832

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