1du0

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{{STRUCTURE_1du0| PDB=1du0 | SCENE= }}
{{STRUCTURE_1du0| PDB=1du0 | SCENE= }}
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'''ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX'''
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===ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX===
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==Overview==
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We have determined the crystal structure of a complex containing the engrailed homeodomain Gln50 --&gt; Ala variant (QA50) bound to the wild-type optimal DNA site (TAATTA) at 2.0 A resolution. Biochemical and genetic studies by other groups have suggested that residue 50 is an important determinant of differential DNA-binding specificity among homeodomains (distinguishing among various sites of the general form TAATNN). However, biochemical studies of the QA50 variant had revealed that it binds almost as tightly as the wild-type protein and with only modest changes in specificity. We have now determined the crystal structure of the QA50 variant to help understand the role of residue 50 in site-specific recognition. Our cocrystal structure shows some interesting changes in the water structure at the site of the substitution and shows some changes in the conformations of neighboring side chains. However, the structure, like the QA50 biochemical data, suggests that Gln50 plays a relatively modest role in determining the affinity and specificity of the engrailed homeodomain.
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{{ABSTRACT_PUBMED_10889025}}
==About this Structure==
==About this Structure==
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[[Category: Homeodomain]]
[[Category: Homeodomain]]
[[Category: Protein-dna complex]]
[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:16:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:36:52 2008''

Revision as of 20:36, 30 June 2008

Image:1du0.png

Template:STRUCTURE 1du0

ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX

Template:ABSTRACT PUBMED 10889025

About this Structure

1DU0 is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

Exploring the role of glutamine 50 in the homeodomain-DNA interface: crystal structure of engrailed (Gln50 --> ala) complex at 2.0 A., Grant RA, Rould MA, Klemm JD, Pabo CO, Biochemistry. 2000 Jul 18;39(28):8187-92. PMID:10889025

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