This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1dv9

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1dv9.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1dv9.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1dv9| PDB=1dv9 | SCENE= }}
{{STRUCTURE_1dv9| PDB=1dv9 | SCENE= }}
-
'''STRUCTURAL CHANGES ACCOMPANYING PH-INDUCED DISSOCIATION OF THE B-LACTOGLOBULIN DIMER'''
+
===STRUCTURAL CHANGES ACCOMPANYING PH-INDUCED DISSOCIATION OF THE B-LACTOGLOBULIN DIMER===
-
==Overview==
+
<!--
-
We have used NMR spectroscopy to determine the three-dimensional (3D) structure, and to characterize the backbone dynamics, of a recombinant version of bovine beta-lactoglobulin (variant A) at pH 2. 6, where the protein is a monomer. The structure of this low-pH form of beta-lactoglobulin is very similar to that of a subunit within the dimer at pH 6.2. The root-mean-square deviation from the pH 6.2 (crystal) structure, calculated for backbone atoms of residues 6-160, is approximately 1.3 A. Differences arise from the orientation, with respect to the calyx, of the A-B and C-D loops, and of the flanking three-turn alpha-helix. The hydrophobic cavity within the calyx is retained at low pH. The E-F loop (residues 85-90), which moves to occlude the opening of the cavity over the pH range 7.2-6.2, is in the "closed" position at pH 2.6, and the side chain of Glu89 is buried. We also carried out measurements of (15)N T(1)s and T(2)s and (1)H-(15)N heteronuclear NOEs at pH 2.6 and 37 degrees C. Although the residues of the E-F loop (residues 86-89) have the highest crystallographic B-factors, the conformation of this loop is reasonably well defined by the NMR data, and its backbone is not especially mobile on the pico- to nanosecond time scale. Several residues (Ser21, Lys60, Ala67, Leu87, and Glu112) exhibit large ratios of T(1) to T(2), consistent with conformational exchange on a micro- to millisecond time scale. The positions of these residues in the 3D structure of beta-lactoglobulin are consistent with a role in modulating access to the hydrophobic cavity.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10736155}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10736155 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10736155}}
==About this Structure==
==About this Structure==
-
1DV9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV9 OCA].
+
1DV9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DV9 OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Low ph structure]]
[[Category: Low ph structure]]
[[Category: Triple resonance experiment]]
[[Category: Triple resonance experiment]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:19:22 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:40:17 2008''

Revision as of 20:40, 30 June 2008

Image:1dv9.png

Template:STRUCTURE 1dv9

STRUCTURAL CHANGES ACCOMPANYING PH-INDUCED DISSOCIATION OF THE B-LACTOGLOBULIN DIMER

Template:ABSTRACT PUBMED 10736155

About this Structure

1DV9 is a Single protein structure of sequence from Bos taurus. Full experimental information is available from OCA.

Reference

Structural changes accompanying pH-induced dissociation of the beta-lactoglobulin dimer., Uhrinova S, Smith MH, Jameson GB, Uhrin D, Sawyer L, Barlow PN, Biochemistry. 2000 Apr 4;39(13):3565-74. PMID:10736155

Page seeded by OCA on Mon Jun 30 23:40:17 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1dv9"
Personal tools