1dvm

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1dvm.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1dvm.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1dvm| PDB=1dvm | SCENE= }}
{{STRUCTURE_1dvm| PDB=1dvm | SCENE= }}
-
'''ACTIVE FORM OF HUMAN PAI-1'''
+
===ACTIVE FORM OF HUMAN PAI-1===
-
==Overview==
+
<!--
-
Serpins exhibit a range of physiological roles and can contribute to certain disease states dependent on their various conformations. Understanding the mechanisms of the large-scale conformational reorganizations of serpins may lead to a better understanding of their roles in various cardiovascular diseases. We have studied the serpin, plasminogen activator inhibitor 1 (PAI-1), in both the active and the latent state and found that anionic halide ions may play a role in the active-to-latent structural transition. Crystallographic analysis of a stable mutant form of active PAI-1 identified an anion-binding site between the central beta-sheet and a small surface domain. A chloride ion was modeled in this site, and its identity was confirmed by soaking crystals in a bromide-containing solution and calculating a crystallographic difference map. The anion thus located forms a 4-fold ligated linchpin that tethers the surface domain to the central beta-sheet into which the reactive center loop must insert during the active-to-latent transition. Timecourse experiments measuring active PAI-1 stability in the presence of various halide ions showed a clear trend for stabilization of the active form with F(-) &gt; Cl(-) &gt; Br(-) &gt;&gt; I(-). We propose that the "stickiness" of this pin (i.e., the electronegativity of the anion) contributes to the energetics of the active-to-latent transition in the PAI-1 serpin.
+
The line below this paragraph, {{ABSTRACT_PUBMED_10913251}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 10913251 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_10913251}}
==About this Structure==
==About this Structure==
Line 29: Line 33:
[[Category: Pai-1]]
[[Category: Pai-1]]
[[Category: Serpin]]
[[Category: Serpin]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:20:09 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:41:16 2008''

Revision as of 20:41, 30 June 2008

Image:1dvm.png

Template:STRUCTURE 1dvm

ACTIVE FORM OF HUMAN PAI-1

Template:ABSTRACT PUBMED 10913251

About this Structure

1DVM is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structures of active and latent PAI-1: a possible stabilizing role for chloride ions., Stout TJ, Graham H, Buckley DI, Matthews DJ, Biochemistry. 2000 Jul 25;39(29):8460-9. PMID:10913251

Page seeded by OCA on Mon Jun 30 23:41:16 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1dvm"
Personal tools