1dz9

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[[Image:1dz9.gif|left|200px]]
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{{STRUCTURE_1dz9| PDB=1dz9 | SCENE= }}
{{STRUCTURE_1dz9| PDB=1dz9 | SCENE= }}
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'''PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA'''
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===PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA===
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==Overview==
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Members of the cytochrome P450 superfamily catalyze the addition of molecular oxygen to nonactivated hydrocarbons at physiological temperature-a reaction that requires high temperature to proceed in the absence of a catalyst. Structures were obtained for three intermediates in the hydroxylation reaction of camphor by P450cam with trapping techniques and cryocrystallography. The structure of the ferrous dioxygen adduct of P450cam was determined with 0.91 angstrom wavelength x-rays; irradiation with 1.5 angstrom x-rays results in breakdown of the dioxygen molecule to an intermediate that would be consistent with an oxyferryl species. The structures show conformational changes in several important residues and reveal a network of bound water molecules that may provide the protons needed for the reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 10698731 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10698731}}
==About this Structure==
==About this Structure==
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[[Category: Mono-oxygenase]]
[[Category: Mono-oxygenase]]
[[Category: Reaction intermediate]]
[[Category: Reaction intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:27:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:51:48 2008''

Revision as of 20:52, 30 June 2008

Image:1dz9.png

Template:STRUCTURE 1dz9

PUTATIVE OXO COMPLEX OF P450CAM FROM PSEUDOMONAS PUTIDA

Template:ABSTRACT PUBMED 10698731

About this Structure

1DZ9 is a Single protein structure of sequence from Pseudomonas putida. Full crystallographic information is available from OCA.

Reference

The catalytic pathway of cytochrome p450cam at atomic resolution., Schlichting I, Berendzen J, Chu K, Stock AM, Maves SA, Benson DE, Sweet RM, Ringe D, Petsko GA, Sligar SG, Science. 2000 Mar 3;287(5458):1615-22. PMID:10698731

Page seeded by OCA on Mon Jun 30 23:51:48 2008

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