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| - | [[Image:1e0b.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1e0b| PDB=1e0b | SCENE= }} | | {{STRUCTURE_1e0b| PDB=1e0b | SCENE= }} |
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| - | '''CHROMO SHADOW DOMAIN FROM FISSION YEAST SWI6 PROTEIN.'''
| + | ===CHROMO SHADOW DOMAIN FROM FISSION YEAST SWI6 PROTEIN.=== |
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| - | ==Overview==
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| - | BACKGROUND: Proteins such as HP1, found in fruit flies and mammals, and Swi6, its fission yeast homologue, carry a chromodomain (CD) and a chromo shadow domain (CSD). These proteins are required to form functional transcriptionally silent centromeric chromatin, and their mutation leads to chromosome segregation defects. CSDs have only been found in tandem in proteins containing the related CD. Most HP1-interacting proteins have been found to associate through the CSD and many of these ligands contain a conserved pentapeptide motif. RESULTS: The 1.9 A crystal structure of the Swi6 CSD is presented here. This reveals a novel dimeric structure that is distinct from the previously reported monomeric nuclear magnetic resonance (NMR) structure of the CD from the mouse modifier 1 protein (MoMOD1, also known as HP1beta or M31). A prominent pit with a non-polar base is generated at the dimer interface, and is commensurate with binding an extended pentapeptide motif. Sequence alignments based on this structure highlight differences between CDs and CSDs that are superimposed on a common structural core. The analyses also revealed a previously unrecognised circumferential hydrophobic sash around the surface of the CD structure. CONCLUSIONS: Dimerisation through the CSD of HP1-like proteins results in the simultaneous formation of a putative protein-protein interaction pit, providing a potential means of targeting CSD-containing proteins to particular chromatin sites.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10801440}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10801440 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10801440}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Shadow]] | | [[Category: Shadow]] |
| | [[Category: Swi6]] | | [[Category: Swi6]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:29:57 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jun 30 23:55:32 2008'' |
Revision as of 20:55, 30 June 2008
Template:STRUCTURE 1e0b
CHROMO SHADOW DOMAIN FROM FISSION YEAST SWI6 PROTEIN.
Template:ABSTRACT PUBMED 10801440
About this Structure
1E0B is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
Reference
Dimerisation of a chromo shadow domain and distinctions from the chromodomain as revealed by structural analysis., Cowieson NP, Partridge JF, Allshire RC, McLaughlin PJ, Curr Biol. 2000 May 4;10(9):517-25. PMID:10801440
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