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1e4p

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[[Image:1e4p.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1e4p| PDB=1e4p | SCENE= }}
{{STRUCTURE_1e4p| PDB=1e4p | SCENE= }}
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'''STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE'''
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===STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE===
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==Overview==
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The cleavage site of the Neurospora VS RNA ribozyme is located in a separate hairpin domain containing a hexanucleotide internal loop with an A-C mismatch and two adjacent G-A mismatches. The solution structure of the internal loop and helix la of the ribozyme substrate hairpin has been determined by nuclear magnetic resonance (NMR) spectroscopy. The 2 nt in the internal loop, flanking the cleavage site, a guanine and adenine, are involved in two sheared G.A base pairs similar to the magnesium ion-binding site of the hammerhead ribozyme. Adjacent to the tandem G.A base pairs, the adenine and cytidine, which are important for cleavage, form a noncanonical wobble A+-C base pair. The dynamic properties of the internal loop and details of the high-resolution structure support the view that the hairpin structure represents a ground state, which has to undergo a conformational change prior to cleavage. Results of chemical modification and mutagenesis data of the Neurospora VS RNA ribozyme can be explained in context with the present three-dimensional structure.
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The line below this paragraph, {{ABSTRACT_PUBMED_11142381}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11142381 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11142381}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E4P OCA].
==Reference==
==Reference==
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[[Category: Rna ribozyme]]
[[Category: Rna ribozyme]]
[[Category: Substrate hairpin]]
[[Category: Substrate hairpin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:39:32 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:08:43 2008''

Revision as of 21:08, 30 June 2008

Image:1e4p.png

Template:STRUCTURE 1e4p

STRUCTURE OF THE RIBOZYME SUBSTRATE HAIRPIN OF NEUROSPORA VS RNA. A CLOSE LOOK AT THE CLEAVAGE SITE

Template:ABSTRACT PUBMED 11142381

About this Structure

Full experimental information is available from OCA.

Reference

Structure of the ribozyme substrate hairpin of Neurospora VS RNA: a close look at the cleavage site., Michiels PJ, Schouten CH, Hilbers CW, Heus HA, RNA. 2000 Dec;6(12):1821-32. PMID:11142381

Page seeded by OCA on Tue Jul 1 00:08:43 2008

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