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1e65

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[[Image:1e65.gif|left|200px]]
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{{STRUCTURE_1e65| PDB=1e65 | SCENE= }}
{{STRUCTURE_1e65| PDB=1e65 | SCENE= }}
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'''AZURIN FROM PSEUDOMONAS AERUGINOSA, APO FORM'''
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===AZURIN FROM PSEUDOMONAS AERUGINOSA, APO FORM===
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==Overview==
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The 3D structure of apo-azurin from Pseudomonas aeruginosa has been determined at 1.85 A resolution. The crystal structure is composed of two different molecular forms of apo-azurin arranged as hetero-dimers in the tetramer of the asymmetric unit. Form 1 closely resembles the holo-protein lacking copper. Form 2 shows differences in the metal binding site region induced by the incorporation of a solvent molecule into this site. The positions of the copper ligands His46 and His117 are shifted by 0.6 A and 1.6 A. The His117 side chain adopts a position at the surface of the protein, thereby facilitating access to the copper site. The presence of two different molecular forms of apo-azurin in the crystal lattice may reflect an equilibrium between the two forms in solution. 1H-NMR spectra of apo-azurin recorded as a function of pH show that at high pH the line broadening of His35, His46 and His117 resonances is consistent with an interconversion between forms 1 and 2. At low pH, no broadening is observed. This may indicate that here the interconversion is fast on the NMR timescale.
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The line below this paragraph, {{ABSTRACT_PUBMED_1633865}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 1633865 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1633865}}
==About this Structure==
==About this Structure==
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[[Category: Messerschmidt, A.]]
[[Category: Messerschmidt, A.]]
[[Category: Nar, H.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:42:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:12:48 2008''

Revision as of 21:12, 30 June 2008

Image:1e65.png

Template:STRUCTURE 1e65

AZURIN FROM PSEUDOMONAS AERUGINOSA, APO FORM

Template:ABSTRACT PUBMED 1633865

About this Structure

1E65 is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.

Reference

Crystal structure of Pseudomonas aeruginosa apo-azurin at 1.85 A resolution., Nar H, Messerschmidt A, Huber R, van de Kamp M, Canters GW, FEBS Lett. 1992 Jul 20;306(2-3):119-24. PMID:1633865

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