1e8l

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1e8l.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1e8l.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1e8l| PDB=1e8l | SCENE= }}
{{STRUCTURE_1e8l| PDB=1e8l | SCENE= }}
-
'''NMR SOLUTION STRUCTURE OF HEN LYSOZYME'''
+
===NMR SOLUTION STRUCTURE OF HEN LYSOZYME===
-
==Overview==
+
<!--
-
A high resolution NMR structure of hen lysozyme has been determined using 209 residual 1H-15N dipolar coupling restraints from measurements made in two different dilute liquid crystalline phases (bicelles) in conjunction with a data set of 1632 NOE distance restraints, 110 torsion angle restraints, and 60 hydrogen bond restraints. The ensemble of 50 low-energy calculated structures has an average backbone RMSD of 0.50+/-0.13A to the mean structure and of 1.49+/-0.10A to the crystal structure of hen lysozyme. To assess the importance of the dipolar coupling data in the structure determination, the final structures are compared with an ensemble calculated using an identical protocol but excluding the dipolar coupling restraints. The comparison shows that structures calculated with the dipolar coupling data are more similar to the crystal structure than those calculated without, and have better stereochemical quality. The structures also show improved quality factors when compared with additional dipolar coupling data that were not included in the structure calculations, with orientation-dependent 15N chemical shift changes measured in the bicelle solutions, and with T1/T2 values obtained from 15N relaxation measurements. Analysis of the ensemble of NMR structures and comparisons with crystal structures, 15N relaxation data, and molecular dynamics simulations of hen lysozyme provides a detailed description of the solution structure of this protein and insights into its dynamical behavior.
+
The line below this paragraph, {{ABSTRACT_PUBMED_11274458}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 11274458 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_11274458}}
==About this Structure==
==About this Structure==
-
1E8L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hwa 1hwa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8L OCA].
+
1E8L is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1hwa 1hwa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E8L OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Spencer, A.]]
[[Category: Spencer, A.]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:47:53 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:19:25 2008''

Revision as of 21:19, 30 June 2008

Image:1e8l.png

Template:STRUCTURE 1e8l

NMR SOLUTION STRUCTURE OF HEN LYSOZYME

Template:ABSTRACT PUBMED 11274458

About this Structure

1E8L is a Single protein structure of sequence from Gallus gallus. This structure supersedes the now removed PDB entry 1hwa. Full experimental information is available from OCA.

Reference

A refined solution structure of hen lysozyme determined using residual dipolar coupling data., Schwalbe H, Grimshaw SB, Spencer A, Buck M, Boyd J, Dobson CM, Redfield C, Smith LJ, Protein Sci. 2001 Apr;10(4):677-88. PMID:11274458

Page seeded by OCA on Tue Jul 1 00:19:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1e8l"
Personal tools