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1eal

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{{STRUCTURE_1eal| PDB=1eal | SCENE= }}
{{STRUCTURE_1eal| PDB=1eal | SCENE= }}
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'''NMR STUDY OF ILEAL LIPID BINDING PROTEIN'''
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===NMR STUDY OF ILEAL LIPID BINDING PROTEIN===
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==Overview==
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BACKGROUND: The family of lipid binding proteins (LBPs) includes a large number of fatty acid binding proteins (FABPs) but only two proteins (ileal lipid binding protein, ILBP, and liver fatty acid binding protein) that can bind both fatty acids and bile acids. Bile acid transport is medically and pharmacologically important, but is poorly understood. To understand the binding properties of ILBP, we studied its solution structure with and without bound lipids and compared these with known structures of FABPs. RESULTS: The sequence-specific 1H resonance assignments for porcine ILBP have been determined by homonuclear two-dimensional (2D) NMR spectroscopy for the apo-protein as well as for ILBP complexes with fatty acid and bile acid ligands. From NOE spectra and hydrogen exchange data, similar secondary structure elements were identified for all three protein forms. ILBP is composed of ten antiparallel beta strands arranged in two nearly orthogonal beta sheets (a fold seen in other FABPs, and dubbed the "beta-clam shell'), covered on one side by two short, nearly parallel alpha helices. Binding of fatty acids or bile acids to ILBP alters mainly the side-chain proton resonances of amino acids within the protein cavity, indicating that both bile acids and fatty acids can bind in the interior of the protein between the two beta sheets; binding of bile acids stabilizes the protein backbone by a small amount. Fast hydrogen exchange rates for the backbone amide protons of ILBP indicate that the hydrogen-bonding network of the beta sheet in ILBP is weaker than the corresponding network in rat intestinal and bovine heart FABPs. CONCLUSIONS: The tertiary structure of ILBP is similar to that of other LBPs, but appears to be unusually flexible, with a relatively weak hydrogen-bonding network. It is likely that this flexibility is important in allowing bile acids, which are larger and more rigid than fatty acids, to enter the central cavity of the protein.
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(as it appears on PubMed at http://www.pubmed.gov), where 8805562 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8805562}}
==About this Structure==
==About this Structure==
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1EAL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAL OCA].
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1EAL is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EAL OCA].
==Reference==
==Reference==
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[[Category: Ileal epithelium]]
[[Category: Ileal epithelium]]
[[Category: Intracellular lipid binding protein]]
[[Category: Intracellular lipid binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:52:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:24:43 2008''

Revision as of 21:24, 30 June 2008

Image:1eal.png

Template:STRUCTURE 1eal

NMR STUDY OF ILEAL LIPID BINDING PROTEIN

Template:ABSTRACT PUBMED 8805562

About this Structure

1EAL is a Single protein structure of sequence from Sus scrofa. Full experimental information is available from OCA.

Reference

Flexibility is a likely determinant of binding specificity in the case of ileal lipid binding protein., Lucke C, Zhang F, Ruterjans H, Hamilton JA, Sacchettini JC, Structure. 1996 Jul 15;4(7):785-800. PMID:8805562

Page seeded by OCA on Tue Jul 1 00:24:43 2008

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