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| | {{STRUCTURE_1ebs| PDB=1ebs | SCENE= }} | | {{STRUCTURE_1ebs| PDB=1ebs | SCENE= }} |
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| - | '''STRUCTURE OF RNA (5'-GGUGGGCGCAGCUUCGGCUGCGGUACCAC-3'), NMR, 5 STRUCTURES'''
| + | ===STRUCTURE OF RNA (5'-GGUGGGCGCAGCUUCGGCUGCGGUACCAC-3'), NMR, 5 STRUCTURES=== |
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| - | ==Overview==
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| - | The HIV-1 Rev responsive element (RRE) high-affinity binding site was studied by homonuclear and heteronuclear NMR. Two Rev binding element (RBE) RNA oligonucleotides were used as model systems in this study: RBE3, which contains the wild-type Rev high-affinity binding site, and RBE3-A which is identical except for the deletion of a bulged A. The temperature dependence of the two-dimensional spectra of the free RNAs indicates that at lower temperatures more than one conformation is present. However, at higher temperatures a single conformation predominates. Model structures of RBE3 and RBE3-A as well as the RBE3-A complexed with a peptide derived from the RNA binding domain of HIV-1 Rev, were calculated using NMR-derived restraints. The Rev high-affinity binding site of the HIV-1 RRE contains a structured internal loop with two purine-purine base-pairs and an extrahelical U. Comparison of the free and bound RNA structures reveals that upon peptide binding there is a distinct change in the backbone at G24, which is involved in a G-G base-pair. In the free RNA, G24 is in the syn conformation, and the backbone is in a relatively normal configuration, antiparallel to the other strand. In the bound RNA, the backbone at G24 has flipped over so that it is parallel to the other strand. G24 in the bound RNA still forms a base-pair with G6, but is now in the anti conformation. | + | The line below this paragraph, {{ABSTRACT_PUBMED_9000617}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 9000617 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_9000617}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBS OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EBS OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Peterson, R D.]] | | [[Category: Peterson, R D.]] |
| | [[Category: Ribonucleic acid]] | | [[Category: Ribonucleic acid]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:54:52 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:27:55 2008'' |
Revision as of 21:27, 30 June 2008
Template:STRUCTURE 1ebs
STRUCTURE OF RNA (5'-GGUGGGCGCAGCUUCGGCUGCGGUACCAC-3'), NMR, 5 STRUCTURES
Template:ABSTRACT PUBMED 9000617
About this Structure
Full experimental information is available from OCA.
Reference
Structural change in Rev responsive element RNA of HIV-1 on binding Rev peptide., Peterson RD, Feigon J, J Mol Biol. 1996 Dec 20;264(5):863-77. PMID:9000617
Page seeded by OCA on Tue Jul 1 00:27:55 2008
