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1eci

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[[Image:1eci.jpg|left|200px]]
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{{STRUCTURE_1eci| PDB=1eci | SCENE= }}
{{STRUCTURE_1eci| PDB=1eci | SCENE= }}
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'''ECTATOMIN (WATER SOLUTION, NMR 20 STRUCTURES)'''
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===ECTATOMIN (WATER SOLUTION, NMR 20 STRUCTURES)===
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==Overview==
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Two-dimensional 1H NMR techniques were used to determine the spatial structure of ectatomin, a toxin from the venom of the ant Ectatomma tuberculatum. Nearly complete proton resonance assignments for two chains of ectatomin (37 and 34 amino acid residues, respectively) were obtained using 2D TOCSY, DQF-COSY and NOESY experiments. The cross-peak volumes in NOESY spectra were used to define the local structure of the protein and generate accurate proton-proton distance constraints employing the MARDIGRAS program. Disulfide bonds were located by analyzing the global fold of ectatomin, calculated with the distance geometry program DIANA. These data, combined with data on the rate of exchange of amide protons with deuterium, were used to obtain a final set of 20 structures by DIANA. These structures were refined by unrestrained energy minimization using the CHARMm program. The resulting rms deviations over 20 structures (excluding the mobile N- and c-termini of each chain) are 0.75 A for backbone heavy atoms, and 1.25 A for all heavy atoms. The conformations of the two chains are similar. Each chain consists of two alpha-helices and a hinge region of four residues; this forms a hairpin structure which is stabilized by disulfide bridges. The hinge regions of the two chains are connected together by a third disulfide bridge. Thus, ectatomin forms a four-alpha-helical bundle structure.
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{{ABSTRACT_PUBMED_7881269}}
==About this Structure==
==About this Structure==
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1ECI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Ectatomma_tuberculatum Ectatomma tuberculatum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECI OCA].
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1ECI is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Ectatomma_tuberculatum Ectatomma tuberculatum]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ECI OCA].
==Reference==
==Reference==
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[[Category: Ant venom]]
[[Category: Ant venom]]
[[Category: Pore-forming toxin]]
[[Category: Pore-forming toxin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:29:43 2008''

Revision as of 21:29, 30 June 2008

Image:1eci.png

Template:STRUCTURE 1eci

ECTATOMIN (WATER SOLUTION, NMR 20 STRUCTURES)

Template:ABSTRACT PUBMED 7881269

About this Structure

1ECI is a Protein complex structure of sequences from Ectatomma tuberculatum. Full experimental information is available from OCA.

Reference

Three-dimensional structure of ectatomin from Ectatomma tuberculatum ant venom., Nolde DE, Sobol AG, Pluzhnikov KA, Grishin EV, Arseniev AS, J Biomol NMR. 1995 Jan;5(1):1-13. PMID:7881269

Page seeded by OCA on Tue Jul 1 00:29:43 2008

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