1ef0

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{{STRUCTURE_1ef0| PDB=1ef0 | SCENE= }}
{{STRUCTURE_1ef0| PDB=1ef0 | SCENE= }}
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'''CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR'''
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===CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR===
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==Overview==
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PI-SceI is a member of a class of proteins (inteins) that excise themselves from a precursor protein and in the process ligate the flanking protein sequences (exteins). We report here the 2.1-A resolution crystal structure of a PI-SceI miniprecursor (VMA29) containing 10 N-terminal extein residues and 4 C-terminal extein residues. Mutations at the N- and C-terminal splicing junctions, blocking in vivo protein splicing, allowed the miniprecursor to be purified and crystallized. The structure reveals both the N- and C-terminal scissile peptide bonds to be in distorted trans conformations (tau approximately 100 degrees ). Modeling of the wild-type PI-SceI based on the VMA29 structure indicates a large conformational change (movement of &gt;9 A) must occur to allow transesterification to be completed. A zinc atom was discovered at the C-terminal splicing junction. Residues Cys(455), His(453), and Glu(80) along with a water molecule (Wat(53)) chelate the zinc atom. The crystal structure of VMA29 has captured the intein in its pre-spliced state.
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(as it appears on PubMed at http://www.pubmed.gov), where 10828056 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10828056}}
==About this Structure==
==About this Structure==
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[[Category: Mini-precursor]]
[[Category: Mini-precursor]]
[[Category: Protein splicing]]
[[Category: Protein splicing]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:01:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:35:54 2008''

Revision as of 21:35, 30 June 2008

Image:1ef0.png

Template:STRUCTURE 1ef0

CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR

Template:ABSTRACT PUBMED 10828056

About this Structure

1EF0 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structural insights into the protein splicing mechanism of PI-SceI., Poland BW, Xu MQ, Quiocho FA, J Biol Chem. 2000 Jun 2;275(22):16408-13. PMID:10828056

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