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1eg1

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{{STRUCTURE_1eg1| PDB=1eg1 | SCENE= }}
{{STRUCTURE_1eg1| PDB=1eg1 | SCENE= }}
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'''ENDOGLUCANASE I FROM TRICHODERMA REESEI'''
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===ENDOGLUCANASE I FROM TRICHODERMA REESEI===
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==Overview==
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Cellulose is the most abundant polymer in the biosphere. Although generally resistant to degradation, it may be hydrolysed by cellulolytic organisms that have evolved a variety of structurally distinct enzymes, cellobiohydrolases and endoglucanases, for this purpose. Endoglucanase I (EG I) is the major endoglucanase produced by the cellulolytic fungus Trichoderma reesei, accounting for 5 to 10% of the total amount of cellulases produced by this organism. Together with EG I from Humicola insolens and T. reesei cellobiohydrolase I (CBH I), the enzyme is classified into family 7 of the glycosyl hydrolases, and it catalyses hydrolysis with a net retention of the anomeric configuration.The structure of the catalytic core domain (residues 1 to 371) of EG I from T. reesei has been determined at 3.6 A resolution by the molecular replacement method using the structures of T. reesei CBH I and H. insolens EG I as search models. By employing the 2-fold non-crystallographic symmetry (NCS), the structure was refined successfully, despite the limited resolution. The final model has an R-factor of 0.201 (Rfree 0.258).The structure of EG I reveals an extended, open substrate-binding cleft, rather than a tunnel as found in the homologous cellobiohydrolase CBH I. This confirms the earlier proposal that the tunnel-forming loops in CBH I have been deleted in EG I, which has resulted in an open active site in EG I, enabling it to function as an endoglucanase. Comparison of the structure of EG I with several related enzymes reveals structural similarities, and differences that relate to their biological function in degrading particular substrates. A possible structural explanation of the drastically different pH profiles of T. reesei and H. insolens EG I is proposed.
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(as it appears on PubMed at http://www.pubmed.gov), where 9325098 is the PubMed ID number.
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{{ABSTRACT_PUBMED_9325098}}
==About this Structure==
==About this Structure==
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[[Category: Endoglucanase]]
[[Category: Endoglucanase]]
[[Category: Mutation]]
[[Category: Mutation]]
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Revision as of 21:38, 30 June 2008

Image:1eg1.png

Template:STRUCTURE 1eg1

ENDOGLUCANASE I FROM TRICHODERMA REESEI

Template:ABSTRACT PUBMED 9325098

About this Structure

1EG1 is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

The crystal structure of the catalytic core domain of endoglucanase I from Trichoderma reesei at 3.6 A resolution, and a comparison with related enzymes., Kleywegt GJ, Zou JY, Divne C, Davies GJ, Sinning I, Stahlberg J, Reinikainen T, Srisodsuk M, Teeri TT, Jones TA, J Mol Biol. 1997 Sep 26;272(3):383-97. PMID:9325098

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