1egu

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[[Image:1egu.gif|left|200px]]
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{{STRUCTURE_1egu| PDB=1egu | SCENE= }}
{{STRUCTURE_1egu| PDB=1egu | SCENE= }}
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'''CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION'''
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===CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION===
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==Overview==
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Streptococcus pneumoniae hyaluronate lyase (spnHL) is a pathogenic bacterial spreading factor and cleaves hyaluronan, an important constituent of the extra- cellular matrix of connective tissues, through an enzymatic beta-elimination process, different from the hyaluronan degradation by hydrolases in animals. The mechanism of hyaluronan binding and degradation was proposed based on the 1.56 A resolution crystal structure, substrate modeling and mutagenesis studies on spnHL. Five mutants, R243V, N349A, H399A, Y408F and N580G, were constructed and their activities confirmed our mechanism hypothesis. The important roles of Tyr408, Asn349 and His399 in enzyme catalysis were proposed, explained and confirmed by mutant studies. The remaining weak enzymatic activity of the H399A mutant, the role of the free carboxylate group on the glucuronate residue, the enzymatic behavior on chondroitin and chondroitin sulfate, and the small activity increase in the N580G mutant were explained based on this mechanism. A possible function of the C-terminal beta-sheet domain is to modulate enzyme activity through binding to calcium ions.
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(as it appears on PubMed at http://www.pubmed.gov), where 10716923 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10716923}}
==About this Structure==
==About this Structure==
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[[Category: Lamani, E.]]
[[Category: Lamani, E.]]
[[Category: Li, S.]]
[[Category: Li, S.]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:05:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:40:45 2008''

Revision as of 21:40, 30 June 2008

Image:1egu.png

Template:STRUCTURE 1egu

CRYSTAL STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE AT 1.56 A RESOLUTION

Template:ABSTRACT PUBMED 10716923

About this Structure

1EGU is a Single protein structure of sequence from Streptococcus pneumoniae. Full crystallographic information is available from OCA.

Reference

Structural basis of hyaluronan degradation by Streptococcus pneumoniae hyaluronate lyase., Li S, Kelly SJ, Lamani E, Ferraroni M, Jedrzejas MJ, EMBO J. 2000 Mar 15;19(6):1228-40. PMID:10716923

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