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| - | [[Image:1ehw.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1ehw| PDB=1ehw | SCENE= }} | | {{STRUCTURE_1ehw| PDB=1ehw | SCENE= }} |
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| - | '''HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4'''
| + | ===HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4=== |
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| - | ==Overview==
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| - | We demonstrate here the catalytic activity and subcellular localization of the Nm23-H4 protein, product of nm23-H4, a new member of the human nm23/nucleoside diphosphate (NDP) kinase gene family (Milon, L., Rousseau-Merck, M., Munier, A., Erent, M., Lascu, I., Capeau, J., and Lacombe, M. L. (1997) Hum. Genet. 99, 550-557). Nm3-H4 was synthesized in escherichia coli as the full-length protein and as a truncated form missing the N-terminal extension characteristic of mitochondrial targeting. The truncated form possesses NDP kinase activity, whereas the full-length protein is inactive, suggesting that the extension prevents enzyme folding and/or activity. X-ray crystallographic analysis was performed on active truncated Nm23-H4. Like other eukaryotic NDP kinases, it is a hexamer. Nm23-H4 naturally possesses a serine residue at position 129, equivalent to the K-pn mutation of the Drosophila NDP kinase. The x-ray structure shows that the presence of Ser(129) has local structural effects that weaken subunit interactions. Site-directed mutagenesis shows that the serine is responsible for the lability of Nm23-H4 to heat and urea treatment, because the S129P mutant is greatly stabilized. Examination of human embryonic kidney 293 cells transfected with green fluorescent protein fusions by confocal microscopy shows a specific mitochondrial localization of Nm23-H4 that was also demonstrated by Western blot analysis of subcellular fractions of these cells. Import into mitochondria is accompanied by cleavage of the N-terminal extension that results in NDP kinase activity. Submitochondrial fractionation indicates that Nm23-H4 is associated with mitochondrial membranes, possibly to the contact sites between the outer and inner membranes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10799505}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10799505 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10799505}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Nm23]] | | [[Category: Nm23]] |
| | [[Category: Nucleoside diphosphate kinase]] | | [[Category: Nucleoside diphosphate kinase]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:07:11 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:43:11 2008'' |
Revision as of 21:43, 30 June 2008
Template:STRUCTURE 1ehw
HUMAN NUCLEOSIDE DIPHOSPHATE KINASE 4
Template:ABSTRACT PUBMED 10799505
About this Structure
1EHW is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase., Milon L, Meyer P, Chiadmi M, Munier A, Johansson M, Karlsson A, Lascu I, Capeau J, Janin J, Lacombe ML, J Biol Chem. 2000 May 12;275(19):14264-72. PMID:10799505
Page seeded by OCA on Tue Jul 1 00:43:11 2008
Categories: Homo sapiens | Nucleoside-diphosphate kinase | Single protein | Capeau, J. | Chiadmi, M. | Janin, J. | Johansson, M. | Karlsson, A. | Lacombe, M-L. | Lascu, I. | Meyer, P. | Milon, L. | Munier, A. | Killer-of-prune | Mitochondrial | Nm23 | Nucleoside diphosphate kinase
