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1eir

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[[Image:1eir.jpg|left|200px]]
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{{STRUCTURE_1eir| PDB=1eir | SCENE= }}
{{STRUCTURE_1eir| PDB=1eir | SCENE= }}
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'''2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE'''
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===2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE===
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==Overview==
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BphC derived from Pseudomonas sp. strain KKS102, an extradiol type catecholic dioxygenase, is a non-heam iron-containing enzyme, playing an important role in the degradation of biphenyl/PCB (Poly Chlorinated Biphenyls) in the microbe. Although we had earlier solved the crystal structure of KKS102 BphC, it was the inactive form with Fe(III) in the active site. In order to determine the active form structure, BphC was re-activated by anaerobic incubation with Fe(II) and ascorbate, and crystallized anaerobically. The crystal structures of activated BphC and its substrate complex (E x S complex) were determined at 2.0 A resolution under cryogenic condition. In addition, crystal structures of unactivated BphC in substrate free and complex forms were also re-determined. Comparison of activated and unactivated E x S complexes reveals that the orientation of the bound substrate in the active site is significantly different between the two. The structural comparison of the substrate free and complex forms of activated BphC show certain small conformational shifts around the active site upon substrate binding. As a result of the conformational shifts, His194, which has been suggested as the catalytic base, takes part in a weak hydrogen bond with hydroxyl group of the substrate.
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(as it appears on PubMed at http://www.pubmed.gov), where 11293547 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11293547}}
==About this Structure==
==About this Structure==
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[[Category: Senda, T.]]
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[[Category: Four repetitions of beta-alpha-beta-beta-beta motif]]
[[Category: Four repetitions of beta-alpha-beta-beta-beta motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:09:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:48:20 2008''

Revision as of 21:48, 30 June 2008

Image:1eir.png

Template:STRUCTURE 1eir

2,3-DIHYDROXYBIPHENYL-1,2-DIOXYGENASE

Template:ABSTRACT PUBMED 11293547

About this Structure

1EIR is a Single protein structure of sequence from Pseudomonas sp.. Full crystallographic information is available from OCA.

Reference

Crystal structures of substrate free and complex forms of reactivated BphC, an extradiol type ring-cleavage dioxygenase., Uragami Y, Senda T, Sugimoto K, Sato N, Nagarajan V, Masai E, Fukuda M, Mitsu Y, J Inorg Biochem. 2001 Feb;83(4):269-79. PMID:11293547

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