1ejb

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{{STRUCTURE_1ejb| PDB=1ejb | SCENE= }}
{{STRUCTURE_1ejb| PDB=1ejb | SCENE= }}
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'''LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE'''
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===LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE===
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==Overview==
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Lumazine synthase of Saccharomyces cerevisiae is a homopentamer with a molecular weight of 90 kDa. Crystals of the recombinant enzyme with a size of up to 1.6 mm were obtained. The space group is P4(1)2(1)2 with lattice dimensions 82.9 A x 82.9 A x 300.2 A. X-ray diffraction data collected under cryogenic conditions were complete to 1.85 A resolution. The structure of the enzyme in complex with the intermediate analogue, 5-(6-D-ribitylamino-2,4-dihydroxypyrimidine-5-yl)-1-pentyl-p hosphonic acid was solved via molecular replacement using the structure of the Bacillus subtilis enzyme as search model and was refined to a final R-factor of 19.8% (Rfree: 22.5%). The conformation of the active site ligand of the enzyme mimicks that of the Schiff base intermediate of the enzyme-catalyzed reaction. The data enable the reconstruction of the reactant topology during the early steps of the catalytic reaction. Structural determinants, which are likely to be responsible for the inability of the S. cerevisiae enzyme to form icosahedral capsids, will be discussed.
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{{ABSTRACT_PUBMED_10860731}}
==About this Structure==
==About this Structure==
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[[Category: Vitamin biosynthesis]]
[[Category: Vitamin biosynthesis]]
[[Category: X-ray structure analysis]]
[[Category: X-ray structure analysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:10:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 00:50:19 2008''

Revision as of 21:50, 30 June 2008

Image:1ejb.png

Template:STRUCTURE 1ejb

LUMAZINE SYNTHASE FROM SACCHAROMYCES CEREVISIAE

Template:ABSTRACT PUBMED 10860731

About this Structure

1EJB is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

The atomic structure of pentameric lumazine synthase from Saccharomyces cerevisiae at 1.85 A resolution reveals the binding mode of a phosphonate intermediate analogue., Meining W, Mortl S, Fischer M, Cushman M, Bacher A, Ladenstein R, J Mol Biol. 2000 May 26;299(1):181-97. PMID:10860731

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