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1eoo

From Proteopedia

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[[Image:1eoo.gif|left|200px]]
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{{Seed}}
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[[Image:1eoo.png|left|200px]]
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{{STRUCTURE_1eoo| PDB=1eoo | SCENE= }}
{{STRUCTURE_1eoo| PDB=1eoo | SCENE= }}
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'''ECORV BOUND TO COGNATE DNA'''
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===ECORV BOUND TO COGNATE DNA===
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==Overview==
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Two new high-resolution cocrystal structures of EcoRV endonuclease bound to DNA show that a large variation in DNA-bending angles is sampled in the ground state binary complex. Together with previous structures, these data reveal a contiguous series of protein conformational states delineating a specific trajectory for the induced-fit pathway. Rotation of the DNA-binding domains, together with movements of two symmetry-related helices binding in the minor groove, causes base unstacking at a key base-pair step and propagates structural changes that assemble the active sites. These structures suggest a complex mechanism for DNA bending that depends on forces generated by interacting protein segments, and on selective neutralization of phosphate charges along the inner face of the bent double helix.
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The line below this paragraph, {{ABSTRACT_PUBMED_10801972}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10801972 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10801972}}
==About this Structure==
==About this Structure==
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[[Category: Induced fit]]
[[Category: Induced fit]]
[[Category: Protein-dna recognition]]
[[Category: Protein-dna recognition]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:21:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:20:48 2008''

Revision as of 22:21, 30 June 2008

Image:1eoo.png

Template:STRUCTURE 1eoo

ECORV BOUND TO COGNATE DNA

Template:ABSTRACT PUBMED 10801972

About this Structure

1EOO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystallographic snapshots along a protein-induced DNA-bending pathway., Horton NC, Perona JJ, Proc Natl Acad Sci U S A. 2000 May 23;97(11):5729-34. PMID:10801972

Page seeded by OCA on Tue Jul 1 01:20:48 2008

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