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1eqp

From Proteopedia

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[[Image:1eqp.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1eqp| PDB=1eqp | SCENE= }}
{{STRUCTURE_1eqp| PDB=1eqp | SCENE= }}
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'''EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS'''
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===EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS===
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==Overview==
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In some species of Candida the CUG codon is encoded as serine and not leucine. In the case of the exo-beta-1,3-glucanase from the pathogenic fungus C. albicans there are two such translational events, one in the prepro-leader sequence and the other at residue 64. Overexpression of active mature enzyme in a yeast host indicated that these two positions are tolerant to substitution. By comparing the crystal structure of the recombinant protein with that of the native (presented here), it is seen how either serine or leucine can be accommodated at position 64. Examination of the relatively few solved protein structures from C. albicans indicates that other CUG encoded serines are also found at non-essential surface sites. However such codon usage is rare in C. albicans, in contrast to C. rugosa, with direct implications for respective recombinant protein production.
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(as it appears on PubMed at http://www.pubmed.gov), where 11112513 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11112513}}
==About this Structure==
==About this Structure==
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[[Category: Candida albican]]
[[Category: Candida albican]]
[[Category: Exoglucanase]]
[[Category: Exoglucanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:43:40 2008''

Revision as of 22:43, 30 June 2008

Image:1eqp.png

Template:STRUCTURE 1eqp

EXO-B-(1,3)-GLUCANASE FROM CANDIDA ALBICANS

Template:ABSTRACT PUBMED 11112513

About this Structure

1EQP is a Single protein structure of sequence from Candida albicans. Full crystallographic information is available from OCA.

Reference

Minor structural consequences of alternative CUG codon usage (Ser for Leu) in Candida albicans exoglucanase., Cutfield JF, Sullivan PA, Cutfield SM, Protein Eng. 2000 Oct;13(10):735-8. PMID:11112513

Page seeded by OCA on Tue Jul 1 01:43:40 2008

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