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1erj

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{{STRUCTURE_1erj| PDB=1erj | SCENE= }}
{{STRUCTURE_1erj| PDB=1erj | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1'''
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===CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1===
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==Overview==
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The Tup1-Ssn6 corepressor complex regulates the expression of several sets of genes, including genes that specify mating type in the yeast Saccharomyces cerevisiae. Repression of mating-type genes occurs when Tup1-Ssn6 is brought to the DNA by the Matalpha2 DNA-binding protein and assembled upstream of a- and haploid-specific genes. We have determined the 2.3 A X-ray crystal structure of the C-terminal domain of Tup1 (accesion No. 1ERJ), a 43 kDa fragment that contains seven copies of the WD40 sequence motif and binds to the Matalpha2 protein. Moreover, this portion of the protein can partially substitute for full-length Tup1 in bringing about transcriptional repression. The structure reveals a seven-bladed beta propeller with an N-terminal subdomain that is anchored to the side of the propeller and extends the beta sheet of one of the blades. Point mutations in Tup1 that specifically affect the Tup1-Matalpha2 interaction cluster on one surface of the propeller. We identified regions of Tup1 that are conserved among the fungal Tup1 homologs and may be important in protein-protein interactions with additional components of the Tup1-mediated repression pathways.
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{{ABSTRACT_PUBMED_10856245}}
==About this Structure==
==About this Structure==
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[[Category: Wolberger, C.]]
[[Category: Wolberger, C.]]
[[Category: Beta-propeller]]
[[Category: Beta-propeller]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:26:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:45:58 2008''

Revision as of 22:46, 30 June 2008

Image:1erj.png

Template:STRUCTURE 1erj

CRYSTAL STRUCTURE OF THE C-TERMINAL WD40 DOMAIN OF TUP1

Template:ABSTRACT PUBMED 10856245

About this Structure

1ERJ is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of the C-terminal domain of Tup1, a corepressor of transcription in yeast., Sprague ER, Redd MJ, Johnson AD, Wolberger C, EMBO J. 2000 Jun 15;19(12):3016-27. PMID:10856245

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