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1esh

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[[Image:1esh.gif|left|200px]]
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{{Seed}}
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[[Image:1esh.png|left|200px]]
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{{STRUCTURE_1esh| PDB=1esh | SCENE= }}
{{STRUCTURE_1esh| PDB=1esh | SCENE= }}
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'''THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA'''
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===THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA===
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==Overview==
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The 3' end of brome mosaic virus RNA contains a tRNA-like sequence that directs its RNA synthesis. A stem loop structure in this sequence, stem loop C (SLC), was investigated using NMR, and correlated with its ability to direct RNA synthesis by its replicase. SLC consists of two discrete domains, a flexible stem with an internal loop and a rigid stem containing a 5'-AUA-3' triloop. Efficient RNA synthesis requires the sequence on only one side of the flexible stem and a specific compact conformation of the triloop. A high resolution structure of the triloop places the 5' adenine out in solution, and the 3' adenine within the triloop, held tightly through stacking and unusual hydrogen bonds. This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis.
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The line below this paragraph, {{ABSTRACT_PUBMED_10802741}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10802741 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10802741}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESH OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ESH OCA].
==Reference==
==Reference==
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[[Category: Stem-loop]]
[[Category: Stem-loop]]
[[Category: Triloop]]
[[Category: Triloop]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:28:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 01:49:08 2008''

Revision as of 22:49, 30 June 2008

Image:1esh.png

Template:STRUCTURE 1esh

THE SOLUTION STRUCTURE OF THE STEM LOOP C 5'AUA3' TRILOOP OF BROME MOSAIC VIRUS (+) STRAND RNA

Template:ABSTRACT PUBMED 10802741

About this Structure

Full experimental information is available from OCA.

Reference

RNA motifs that determine specificity between a viral replicase and its promoter., Kim CH, Kao CC, Tinoco I Jr, Nat Struct Biol. 2000 May;7(5):415-23. PMID:10802741

Page seeded by OCA on Tue Jul 1 01:49:08 2008

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