1fhw
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(New page: 200px<br /><applet load="1fhw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fhw, resolution 1.90Å" /> '''Structure of the ple...)
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Revision as of 12:48, 20 November 2007
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Structure of the pleckstrin homology domain from GRP1 in complex with inositol(1,3,4,5,6)pentakisphosphate
Overview
Pleckstrin homology (PH) domains are protein modules of around 120 amino, acids found in many proteins involved in cellular signaling. Certain PH, domains drive signal-dependent membrane recruitment of their host proteins, by binding strongly and specifically to lipid second messengers produced, by agonist-stimulated phosphoinositide 3-kinases (PI 3-Ks). We describe, X-ray crystal structures of two different PH domains bound to, Ins(1,3,4,5)P4, the head group of the major PI 3-K product, PtdIns(3,4,5)P3. One of these PH domains (from Grp1) is PtdIns(3,4,5)P3, specific, while the other (from DAPP1/PHISH) binds strongly to both, PtdIns(3,4,5)P3 and its 5'-dephosphorylation product, PtdIns(3,4)P2., Comparison of the two structures provides an explanation for the distinct, phosphoinositide specificities of the two PH domains and allows us to, predict the 3-phosphoinositide selectivity of uncharacterized PH domains.
About this Structure
1FHW is a Single protein structure of sequence from Mus musculus with SO4 and I5P as ligands. Full crystallographic information is available from OCA.
Reference
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains., Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA, Mol Cell. 2000 Aug;6(2):373-84. PMID:10983984
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