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1ew8

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[[Image:1ew8.gif|left|200px]]
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{{STRUCTURE_1ew8| PDB=1ew8 | SCENE= }}
{{STRUCTURE_1ew8| PDB=1ew8 | SCENE= }}
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'''ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID'''
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===ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID===
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==Overview==
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Two high resolution crystal structures of Escherichia coli alkaline phosphatase (AP) in the presence of phosphonate inhibitors are reported. The phosphonate compounds, phosphonoacetic acid (PAA) and mercaptomethylphosphonic acid (MMP), bind competitively to AP with dissociation constants of 5.5 and 0.6 mM, respectively. The structures of the complexes of AP with PAA and MMP were refined at high resolution to crystallographic R-values of 19.0 and 17.5%, respectively. Refinement of the AP-inhibitor complexes was carried out using X-PLOR. The final round of refinement was done using SHELXL-97. Crystallographic analyses of the inhibitor complexes reveal different binding modes for the two phosphonate compounds. The significant difference in binding constants can be attributed to these alternative binding modes observed in the high resolution X-ray structures. The phosphinyl group of PAA coordinates to the active site zinc ions in a manner similar to the competitive inhibitor and product inorganic phosphate. In contrast, MMP binds with its phosphonate moiety directed toward solvent. Both enzyme-inhibitor complexes exhibit close contacts, one of which has the chemical and geometrical potential to be considered an unconventional hydrogen bond of the type C-H...X.
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(as it appears on PubMed at http://www.pubmed.gov), where 10850800 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10850800}}
==About this Structure==
==About this Structure==
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[[Category: Widlanski, T S.]]
[[Category: Widlanski, T S.]]
[[Category: Enzyme-inhibitor complex]]
[[Category: Enzyme-inhibitor complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:35:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:06:58 2008''

Revision as of 23:06, 30 June 2008

Image:1ew8.png

Template:STRUCTURE 1ew8

ALKALINE PHOSPHATASE (E.C. 3.1.3.1) COMPLEX WITH PHOSPHONOACETIC ACID

Template:ABSTRACT PUBMED 10850800

About this Structure

1EW8 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Alternate modes of binding in two crystal structures of alkaline phosphatase-inhibitor complexes., Holtz KM, Stec B, Myers JK, Antonelli SM, Widlanski TS, Kantrowitz ER, Protein Sci. 2000 May;9(5):907-15. PMID:10850800

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