1exn

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{{STRUCTURE_1exn| PDB=1exn | SCENE= }}
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'''T5 5'-EXONUCLEASE'''
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===T5 5'-EXONUCLEASE===
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==Overview==
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THE 5'-exonucleases are enzymes that are essential for DNA replication and repair. As well as their exonucleolytic action, removing nucleotides from the 5'-end of nucleic acid molecules such as Okazaki fragments, many 5'-3'-exonucleases have been shown to possess endonucleolytic activities. T5 5'-3'-exonuclease shares many similarities with the amino terminal of eubacterial DNA polymerases, although, unlike eubacteria, phages such as T5, T4 and T7 express polymerase and 5'-exonuclease proteins from separate genes. Here we report the 2.5-A crystal structure of the phage T5 5'-exonuclease, which reveals a helical arch for binding DNA. We propose a model consistent with a threading mechanism in which single-stranded DNA could slide through the arch, which is formed by two helices, one containing positively charged, and the other hydrophobic, residues. The active site is at the base of the arch, and contains two metal-binding sites.
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==About this Structure==
==About this Structure==
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[[Category: Hydrolase]]
[[Category: Hydrolase]]
[[Category: Nuclease]]
[[Category: Nuclease]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:10:46 2008''

Revision as of 23:10, 30 June 2008

Image:1exn.png


PDB ID 1exn

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1exn, resolution 2.50Å ()
Non-Standard Residues:
Gene: D15 (Enterobacteria phage T5)
Activity: Exodeoxyribonuclease (lambda-induced), with EC number 3.1.11.3
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



T5 5'-EXONUCLEASE

Template:ABSTRACT PUBMED 8657312

About this Structure

1EXN is a Single protein structure of sequence from Enterobacteria phage t5. Full crystallographic information is available from OCA.

Reference

A helical arch allowing single-stranded DNA to thread through T5 5'-exonuclease., Ceska TA, Sayers JR, Stier G, Suck D, Nature. 1996 Jul 4;382(6586):90-3. PMID:8657312

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