1exy

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[[Image:1exy.gif|left|200px]]
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{{STRUCTURE_1exy| PDB=1exy | SCENE= }}
{{STRUCTURE_1exy| PDB=1exy | SCENE= }}
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'''SOLUTION STRUCTURE OF HTLV-1 PEPTIDE BOUND TO ITS RNA APTAMER TARGET'''
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===SOLUTION STRUCTURE OF HTLV-1 PEPTIDE BOUND TO ITS RNA APTAMER TARGET===
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==Overview==
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BACKGROUND: The Rex protein of the human T cell leukemia virus type 1 (HTLV-1) belongs to a family of proteins that use arginine-rich motifs (ARMs) to recognize their RNA targets. Previously, an in vitro selected RNA aptamer sequence was identified that mediates mRNA transport in vivo when placed in the primary binding site on stem-loop IID of the Rex response element. We present the solution structure of the HTLV-1 arginine-rich Rex peptide bound to its RNA aptamer target determined by multidimensional heteronuclear NMR spectroscopy. RESULTS: The Rex peptide in a predominantly extended conformation threads through a channel formed by the shallow and widened RNA major groove and a looped out guanine. The RNA aptamer contains three stems separated by a pair of two-base bulges, and adopts an unanticipated fold in which both junctional sites are anchored through base triple formation. Binding specificity is associated with intermolecular hydrogen bonding between guanidinium groups of three non-adjacent arginines and the guanine base edges of three adjacent G.C pairs. CONCLUSIONS: The extended S-shaped conformation of the Rex peptide, together with previous demonstrations of a beta-hairpin conformation for the bovine immunodeficiency virus (BIV) Tat peptide and an alpha-helical conformation for the human immunodeficiency virus (HIV) Rev peptide in complex with their respective RNA targets, expands our understanding of the strategies employed by ARMs for adaptive recognition and highlights the importance of RNA tertiary structure in accommodating minimalist elements of protein secondary structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 10647177 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10647177}}
==About this Structure==
==About this Structure==
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1EXY is a [[Single protein]] structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1c4j 1c4j]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EXY OCA].
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1EXY is a [[Single protein]] structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1c4j 1c4j]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EXY OCA].
==Reference==
==Reference==
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[[Category: Junctional base triplet]]
[[Category: Junctional base triplet]]
[[Category: Rna binding pocket architecture]]
[[Category: Rna binding pocket architecture]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:39:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:11:26 2008''

Revision as of 23:11, 30 June 2008

Image:1exy.png

Template:STRUCTURE 1exy

SOLUTION STRUCTURE OF HTLV-1 PEPTIDE BOUND TO ITS RNA APTAMER TARGET

Template:ABSTRACT PUBMED 10647177

About this Structure

1EXY is a Single protein structure. This structure supersedes the now removed PDB entry 1c4j. Full experimental information is available from OCA.

Reference

Anchoring an extended HTLV-1 Rex peptide within an RNA major groove containing junctional base triples., Jiang F, Gorin A, Hu W, Majumdar A, Baskerville S, Xu W, Ellington A, Patel DJ, Structure. 1999 Dec 15;7(12):1461-72. PMID:10647177

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