1fje
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(New page: 200px<br /><applet load="1fje" size="450" color="white" frame="true" align="right" spinBox="true" caption="1fje" /> '''SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COM...)
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Revision as of 12:51, 20 November 2007
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SOLUTION STRUCTURE OF NUCLEOLIN RBD12 IN COMPLEX WITH SNRE RNA
Overview
The structure of the 28 kDa complex of the first two RNA binding domains, (RBDs) of nucleolin (RBD12) with an RNA stem-loop that includes the, nucleolin recognition element UCCCGA in the loop was determined by NMR, spectroscopy. The structure of nucleolin RBD12 with the nucleolin, recognition element (NRE) reveals that the two RBDs bind on opposite sides, of the RNA loop, forming a molecular clamp that brings the 5' and 3' ends, of the recognition sequence close together and stabilizing the stem-loop., The specific interactions observed in the structure explain the sequence, specificity for the NRE sequence. Binding studies of mutant proteins and, analysis of conserved residues support the proposed interactions. The mode, of interaction of the protein with the RNA and the location of the, putative NRE sites suggest that nucleolin may function as an RNA chaperone, to prevent improper folding of the nascent pre-rRNA.
About this Structure
1FJE is a Single protein structure of sequence from Mesocricetus auratus. Full crystallographic information is available from OCA.
Reference
Molecular basis of sequence-specific recognition of pre-ribosomal RNA by nucleolin., Allain FH, Bouvet P, Dieckmann T, Feigon J, EMBO J. 2000 Dec 15;19(24):6870-81. PMID:11118222
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