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1f24

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{{STRUCTURE_1f24| PDB=1f24 | SCENE= }}
{{STRUCTURE_1f24| PDB=1f24 | SCENE= }}
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'''CRYSTAL STRUCTURE OF NO COMPLEX OF THR243ALA MUTANTS OF CYTOCHROME P450NOR'''
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===CRYSTAL STRUCTURE OF NO COMPLEX OF THR243ALA MUTANTS OF CYTOCHROME P450NOR===
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==Overview==
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Threonine 243 of cytochrome P450nor (fungal nitric oxide reductase) corresponds to the 'conserved' Thr in the long I helix of monooxygenase cytochrome P450s. In P450nor, the replacement of Thr243 with Asn, Ala or Val makes the enzymatic activity dramatically reduce. In order to understand the roles of Thr243 in the reduction reaction of NO by P450nor, the crystal structures of three Thr243 mutants (Thr243--&gt;Asn, Thr243--&gt;Val, Thr243--&gt;Ala) of P450nor were determined at a 1.4-A resolution and at cryogenic temperature. However, the hydrogen-bonding pattern in the heme pocket of these mutants is essentially similar for that of the WT enzyme. This suggests that the determination of the structure of the NADH complex of P450nor is required, in order to evaluate the role of Thr243 in its enzymatic reaction. We attempted to crystallize the NADH complex under several conditions, but have not yet been successful.
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(as it appears on PubMed at http://www.pubmed.gov), where 11132616 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11132616}}
==About this Structure==
==About this Structure==
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[[Category: Cytochrome p450nor]]
[[Category: Cytochrome p450nor]]
[[Category: Nitric oxide reductase]]
[[Category: Nitric oxide reductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:47:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:21:37 2008''

Revision as of 23:21, 30 June 2008

Image:1f24.png

Template:STRUCTURE 1f24

CRYSTAL STRUCTURE OF NO COMPLEX OF THR243ALA MUTANTS OF CYTOCHROME P450NOR

Template:ABSTRACT PUBMED 11132616

About this Structure

1F24 is a Single protein structure of sequence from Fusarium oxysporum. Full crystallographic information is available from OCA.

Reference

Mutation effects of a conserved threonine (Thr243) of cytochrome P450nor on its structure and function., Obayashi E, Shimizu H, Park SY, Shoun H, Shiro Y, J Inorg Biochem. 2000 Nov;82(1-4):103-11. PMID:11132616

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