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| | {{STRUCTURE_1f27| PDB=1f27 | SCENE= }} | | {{STRUCTURE_1f27| PDB=1f27 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT'''
| + | ===CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT=== |
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| - | ==Overview==
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| - | A pseudoknot-containing aptamer isolated from a pool of random sequence molecules has been shown previously to represent an optimal RNA solution to the problem of binding biotin. The affinity of this RNA molecule is nonetheless orders of magnitude weaker than that of its highly evolved protein analogs, avidin and streptavidin. To understand the structural basis for biotin binding and to compare directly strategies for ligand recognition available to proteins and RNA molecules, we have determined the 1.3 A crystal structure of the aptamer complexed with its ligand. Biotin is bound at the interface between the pseudoknot's stacked helices in a pocket defined almost entirely by base-paired nucleotides. In comparison to the protein avidin, the aptamer packs more tightly around the biotin headgroup and makes fewer contacts with its fatty acid tail. Whereas biotin is deeply buried within the hydrophobic core in the avidin complex, the aptamer relies on a combination of hydrated magnesium ions and immobilized water molecules to surround its ligand. In addition to demonstrating fundamentally different approaches to molecular recognition by proteins and RNA, the structure provides general insight into the mechanisms by which RNA function is mediated by divalent metals.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_10698630}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 10698630 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_10698630}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Pseudoknot]] | | [[Category: Pseudoknot]] |
| | [[Category: Rna aptamer]] | | [[Category: Rna aptamer]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:47:59 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:21:57 2008'' |
Revision as of 23:21, 30 June 2008
Template:STRUCTURE 1f27
CRYSTAL STRUCTURE OF A BIOTIN-BINDING RNA PSEUDOKNOT
Template:ABSTRACT PUBMED 10698630
About this Structure
Full crystallographic information is available from OCA.
Reference
The 1.3 A crystal structure of a biotin-binding pseudoknot and the basis for RNA molecular recognition., Nix J, Sussman D, Wilson C, J Mol Biol. 2000 Mar 10;296(5):1235-44. PMID:10698630
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