1f3g

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{{STRUCTURE_1f3g| PDB=1f3g | SCENE= }}
{{STRUCTURE_1f3g| PDB=1f3g | SCENE= }}
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'''THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC'''
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===THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC===
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==Overview==
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The crystal structure of a proteolytically modified form of the Escherichia coli phosphocarrier and signal transducing protein IIIglc has been determined by multiple isomorphous and molecular replacement. The model has been refined to an R-factor of 0.166 for data between 6- and 2.1-A resolution with an rms deviation of 0.020 A from ideal bond lengths and 3.2 degrees from ideal bond angles. The molecule is a beta-sheet sandwich, with six antiparallel strands on either side. Several short distorted helices line the periphery of the active site, which is a shallow extremely hydrophobic depression approximately 18 A in diameter near the center of one face. The side chains of the active site histidine residues 75 and 90 face each other at the center of the depression, with the N3 positions exposed to solvent, separated by 3.3 A in an excellent position to form adducts with phosphate. Chloroplatinate forms a divalent adduct with both histidyl side chains, suggesting that the phosphodonor reaction might proceed through a similar transition state. The hydrophobic patch forms the primary crystal contact, suggesting a mode of association of IIIglc with other components of the phosphoenolpyruvate-dependent phosphotransferase system.
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(as it appears on PubMed at http://www.pubmed.gov), where 1961703 is the PubMed ID number.
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{{ABSTRACT_PUBMED_1961703}}
==About this Structure==
==About this Structure==
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[[Category: Worthylake, D.]]
[[Category: Worthylake, D.]]
[[Category: Phosphotransferase]]
[[Category: Phosphotransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:36:38 2008''

Revision as of 23:36, 30 June 2008

Image:1f3g.png

Template:STRUCTURE 1f3g

THREE-DIMENSIONAL STRUCTURE OF THE ESCHERICHIA COLI PHOSPHOCARRIER PROTEIN III GLC

Template:ABSTRACT PUBMED 1961703

About this Structure

1F3G is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Three-dimensional structure of the Escherichia coli phosphocarrier protein IIIglc., Worthylake D, Meadow ND, Roseman S, Liao DI, Herzberg O, Remington SJ, Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10382-6. PMID:1961703

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