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1f43

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[[Image:1f43.gif|left|200px]]
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[[Image:1f43.png|left|200px]]
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{{STRUCTURE_1f43| PDB=1f43 | SCENE= }}
{{STRUCTURE_1f43| PDB=1f43 | SCENE= }}
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'''SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN'''
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===SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN===
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==Overview==
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The mating type homeodomain proteins, MATa1 and MATalpha2, combine to form a heterodimer to bind DNA in diploid yeast cells. The a1-alpha2 heterodimer tightly and specifically binds haploid-specific gene operators to repress transcription. On its own, however, the a1 homeodomain does not bind DNA in a sequence-specific manner. To help understand this interaction, we describe the solution structure and backbone dynamics of the free a1 homeodomain. Free a1 in solution is an ensemble of structures having flexible hinges at the two turns in the small protein fold. Conformational changes in the a1 homeodomain upon ternary complex formation are located in the loop between helix 1 and helix 2, where the C-terminal tail of alpha2 binds to form the heterodimer, and at the C-terminus of helix 3, the DNA recognition helix. The observed differences, comparing the free and bound a1 structures, suggest a mechanism linking van der Waals stacking changes to the ordering of a final turn in the DNA-binding helix of a1. The tail of alpha2 induces changes in loop 1 of a1 that push it toward a properly folded DNA binding conformation.
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The line below this paragraph, {{ABSTRACT_PUBMED_10955992}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 10955992 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10955992}}
==About this Structure==
==About this Structure==
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1F43 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F43 OCA].
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1F43 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1F43 OCA].
==Reference==
==Reference==
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[[Category: Helix-turn-helix]]
[[Category: Helix-turn-helix]]
[[Category: Homeodomain]]
[[Category: Homeodomain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:52:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:39:10 2008''

Revision as of 23:39, 30 June 2008

Image:1f43.png

Template:STRUCTURE 1f43

SOLUTION STRUCTURE OF THE MATA1 HOMEODOMAIN

Template:ABSTRACT PUBMED 10955992

About this Structure

1F43 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full experimental information is available from OCA.

Reference

Cooperative ordering in homeodomain-DNA recognition: solution structure and dynamics of the MATa1 homeodomain., Anderson JS, Forman MD, Modleski S, Dahlquist FW, Baxter SM, Biochemistry. 2000 Aug 22;39(33):10045-54. PMID:10955992

Page seeded by OCA on Tue Jul 1 02:39:10 2008

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