1f4z

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{{STRUCTURE_1f4z| PDB=1f4z | SCENE= }}
{{STRUCTURE_1f4z| PDB=1f4z | SCENE= }}
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'''BACTERIORHODOPSIN-M PHOTOINTERMEDIATE STATE OF THE E204Q MUTANT AT 1.8 ANGSTROM RESOLUTION'''
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===BACTERIORHODOPSIN-M PHOTOINTERMEDIATE STATE OF THE E204Q MUTANT AT 1.8 ANGSTROM RESOLUTION===
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==Overview==
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In order to understand how isomerization of the retinal drives unidirectional transmembrane ion transport in bacteriorhodopsin, we determined the atomic structures of the BR state and M photointermediate of the E204Q mutant, to 1.7 and 1.8 A resolution, respectively. Comparison of this M, in which proton release to the extracellular surface is blocked, with the previously determined M in the D96N mutant indicates that the changes in the extracellular region are initiated by changes in the electrostatic interactions of the retinal Schiff base with Asp85 and Asp212, but those on the cytoplasmic side originate from steric conflict of the 13-methyl retinal group with Trp182 and distortion of the pi-bulge of helix G. The structural changes suggest that protonation of Asp85 initiates a cascade of atomic displacements in the extracellular region that cause release of a proton to the surface. The progressive relaxation of the strained 13-cis retinal chain with deprotonated Schiff base, in turn, initiates atomic displacements in the cytoplasmic region that cause the intercalation of a hydrogen-bonded water molecule between Thr46 and Asp96. This accounts for the lowering of the pK(a) of Asp96, which then reprotonates the Schiff base via a newly formed chain of water molecules that is extending toward the Schiff base.
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(as it appears on PubMed at http://www.pubmed.gov), where 10903866 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10903866}}
==About this Structure==
==About this Structure==
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[[Category: Photoreceptor]]
[[Category: Photoreceptor]]
[[Category: Retinal protein]]
[[Category: Retinal protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:54:24 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:42:06 2008''

Revision as of 23:42, 30 June 2008

Image:1f4z.png

Template:STRUCTURE 1f4z

BACTERIORHODOPSIN-M PHOTOINTERMEDIATE STATE OF THE E204Q MUTANT AT 1.8 ANGSTROM RESOLUTION

Template:ABSTRACT PUBMED 10903866

About this Structure

1F4Z is a Single protein structure of sequence from Halobacterium salinarum. Full crystallographic information is available from OCA.

Reference

Coupling photoisomerization of retinal to directional transport in bacteriorhodopsin., Luecke H, Schobert B, Cartailler JP, Richter HT, Rosengarth A, Needleman R, Lanyi JK, J Mol Biol. 2000 Jul 28;300(5):1237-55. PMID:10903866

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