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1f57

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[[Image:1f57.jpg|left|200px]]
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{{STRUCTURE_1f57| PDB=1f57 | SCENE= }}
{{STRUCTURE_1f57| PDB=1f57 | SCENE= }}
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'''CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A'''
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===CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A===
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==Overview==
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D-Cysteine differs from the antiarthritis drug D-penicillamine by only two methyl groups on the beta-carbon yet inhibits carboxypeptidase A (CPD) by a distinct mechanism: D-cysteine binds tightly to the active site zinc, while D-penicillamine catalyzes metal removal. To investigate the structural basis for this difference, we solved the crystal structure of carboxypeptidase A complexed with D-cysteine (D-Cys) at 1.75-A resolution. D-Cys binds the active site zinc with a sulfur ligand and forms additional interactions with surrounding side chains of the enzyme. The structure explains the difference in potency between D-Cys and L-Cys and provides insight into the mechanism of D-penicillamine inhibition. D-Cys binding induces a concerted motion of the side chains around the zinc ion, similar to that found in other carboxypeptidase-inhibitor crystal structures and along a limited path. Analysis of concerted motions of CPD and CPD-inhibitor crystal structures reveals a clustering of these structures into distinct groups. Using the restricted conformational flexibility of a drug target in this type of analysis could greatly enhance efficiency in drug design.
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(as it appears on PubMed at http://www.pubmed.gov), where 10955996 is the PubMed ID number.
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{{ABSTRACT_PUBMED_10955996}}
==About this Structure==
==About this Structure==
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[[Category: Joshua-Tor, L.]]
[[Category: Joshua-Tor, L.]]
[[Category: Metalloprotease inhibitor]]
[[Category: Metalloprotease inhibitor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:54:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:42:53 2008''

Revision as of 23:42, 30 June 2008

Image:1f57.png

Template:STRUCTURE 1f57

CARBOXYPEPTIDASE A COMPLEX WITH D-CYSTEINE AT 1.75 A

Template:ABSTRACT PUBMED 10955996

About this Structure

1F57 is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Crystal structure of carboxypeptidase A complexed with D-cysteine at 1.75 A - inhibitor-induced conformational changes., van Aalten DM, Chong CR, Joshua-Tor L, Biochemistry. 2000 Aug 22;39(33):10082-9. PMID:10955996

Page seeded by OCA on Tue Jul 1 02:42:53 2008

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