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1f6l

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{{STRUCTURE_1f6l| PDB=1f6l | SCENE= }}
{{STRUCTURE_1f6l| PDB=1f6l | SCENE= }}
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'''VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY'''
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===VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY===
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==Overview==
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The antiferritin variable light domain (VL) dimer binds human spleen ferritin ( approximately 85% L subunits) but with approximately 50-fold lower affinity, K(a)=4 x 10(7) x M(-1), than the parent F11 antibody (K(a)=2.1 x 10(9) x M(-1)). The VL dimer does not recognize either rL (100% L subunits) or rH (100% H subunits) human ferritin, whereas the parent antibody recognizes rL-ferritin. To help explain the differences in ferritin binding affinities and specificities, the crystal structure of the VL domain (2.8A resolution) was determined by molecular replacement and models of the antiferritin VL-VH dimer were made on the basis of antilysozyme antibody D1.3. The domain interface is smaller in the VL dimer but a larger number of interdomain hydrogen bonds may prevent rearrangement on antigen binding. The antigen binding surface of the VL dimer is flatter, lacking a negatively charged pocket found in the VL-VH models, contributed by the CDR3 loop of the VH domain. Loop CDR2 (VL dimer) is located away from the antigen binding site, while the corresponding loop of the VH domain would be located within the antigen binding site. Together these differences lead to 50-fold lower binding affinity in the VL dimer and to more restricted specificity than is seen for the parent antibody.
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(as it appears on PubMed at http://www.pubmed.gov), where 12217656 is the PubMed ID number.
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{{ABSTRACT_PUBMED_12217656}}
==About this Structure==
==About this Structure==
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[[Category: Immunoglobulin fold]]
[[Category: Immunoglobulin fold]]
[[Category: Variable light chain]]
[[Category: Variable light chain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 15:57:54 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 02:47:05 2008''

Revision as of 23:47, 30 June 2008

Image:1f6l.png

Template:STRUCTURE 1f6l

VARIABLE LIGHT CHAIN DIMER OF ANTI-FERRITIN ANTIBODY

Template:ABSTRACT PUBMED 12217656

About this Structure

1F6L is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Antiferritin VL homodimer binds human spleen ferritin with high specificity., Nymalm Y, Kravchuk Z, Salminen T, Chumanevich AA, Dubnovitsky AP, Kankare J, Pentikainen O, Lehtonen J, Arosio P, Martsev S, Johnson MS, J Struct Biol. 2002 Jun;138(3):171-86. PMID:12217656

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