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1fct

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[[Image:1fct.gif|left|200px]]
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{{Seed}}
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[[Image:1fct.png|left|200px]]
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{{STRUCTURE_1fct| PDB=1fct | SCENE= }}
{{STRUCTURE_1fct| PDB=1fct | SCENE= }}
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'''NMR STRUCTURES OF FERREDOXIN CHLOROPLASTIC TRANSIT PEPTIDE FROM CHLAMYDOMONAS REINHARDTII PROMOTED BY TRIFLUOROETHANOL IN AQUEOUS SOLUTION'''
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===NMR STRUCTURES OF FERREDOXIN CHLOROPLASTIC TRANSIT PEPTIDE FROM CHLAMYDOMONAS REINHARDTII PROMOTED BY TRIFLUOROETHANOL IN AQUEOUS SOLUTION===
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==Overview==
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The 32-amino acid transit peptide of the unicellular green alga Chlamydomonas reinhardtii ferredoxin has been synthesized and analysed by NMR spectroscopy and circular dichroism. The results show that while the peptide is unstructured in water, it undergoes an alpha-helix formation from residue 3 to 13 in a 30:70 molar-ratio mixture of 2,2,2-trifluoroethanol. The remainder of the peptide is still unstructured in CF3CD2OD/H2O mixtures, but is distributed on a side opposite to a hydrophobic ridge formed by Met5, Phe9 and Val13 on the induced alpha-helix. The NMR structures driven by 2,2,2-trifluoroethanol in aqueous solution, are discussed in terms of potent interactions with the chloroplast envelope and its translocation molecular machinery.
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The line below this paragraph, {{ABSTRACT_PUBMED_8174712}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 8174712 is the PubMed ID number.
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{{ABSTRACT_PUBMED_8174712}}
==About this Structure==
==About this Structure==
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1FCT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCT OCA].
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1FCT is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1FCT OCA].
==Reference==
==Reference==
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[[Category: Lancelin, J M.]]
[[Category: Lancelin, J M.]]
[[Category: Transit peptide]]
[[Category: Transit peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:10:55 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:03:42 2008''

Revision as of 00:03, 1 July 2008

Image:1fct.png

Template:STRUCTURE 1fct

NMR STRUCTURES OF FERREDOXIN CHLOROPLASTIC TRANSIT PEPTIDE FROM CHLAMYDOMONAS REINHARDTII PROMOTED BY TRIFLUOROETHANOL IN AQUEOUS SOLUTION

Template:ABSTRACT PUBMED 8174712

About this Structure

1FCT is a Single protein structure of sequence from Chlamydomonas reinhardtii. Full experimental information is available from OCA.

Reference

NMR structures of ferredoxin chloroplastic transit peptide from Chlamydomonas reinhardtii promoted by trifluoroethanol in aqueous solution., Lancelin JM, Bally I, Arlaud GJ, Blackledge M, Gans P, Stein M, Jacquot JP, FEBS Lett. 1994 May 2;343(3):261-6. PMID:8174712

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