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1fjo

From Proteopedia

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[[Image:1fjo.jpg|left|200px]]
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{{STRUCTURE_1fjo| PDB=1fjo | SCENE= }}
{{STRUCTURE_1fjo| PDB=1fjo | SCENE= }}
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'''THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)'''
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===THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)===
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==Overview==
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Multiple Solvent Crystal Structures (MSCS) is a crystallographic technique to identify energetically favorable positions and orientations of small organic molecules on the surface of proteins. We determined the high-resolution crystal structures of thermolysin (TLN), generated from crystals soaked in 50--70% acetone, 50--80% acetonitrile and 50 mM phenol. The structures of the protein in the aqueous-organic mixtures are essentially the same as the native enzyme and a number of solvent interaction sites were identified. The distribution of probe molecules shows clusters in the main specificity pocket of the active site and a buried subsite. Within the active site, we compared the experimentally determined solvent positions with predictions from two computational functional group mapping techniques, GRID and Multiple Copy Simultaneous Search (MCSS). The experimentally determined small molecule positions are consistent with the structures of known protein--ligand complexes of TLN.
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(as it appears on PubMed at http://www.pubmed.gov), where 11287678 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11287678}}
==About this Structure==
==About this Structure==
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[[Category: Metalloproteinase]]
[[Category: Metalloproteinase]]
[[Category: Organic solvent]]
[[Category: Organic solvent]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:24:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:22:21 2008''

Revision as of 00:22, 1 July 2008

Image:1fjo.png

Template:STRUCTURE 1fjo

THERMOLYSIN (60% ACETONE SOAKED CRYSTALS)

Template:ABSTRACT PUBMED 11287678

About this Structure

1FJO is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

Reference

Experimental and computational mapping of the binding surface of a crystalline protein., English AC, Groom CR, Hubbard RE, Protein Eng. 2001 Jan;14(1):47-59. PMID:11287678

Page seeded by OCA on Tue Jul 1 03:22:21 2008

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