1fq3

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[[Image:1fq3.gif|left|200px]]
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{{STRUCTURE_1fq3| PDB=1fq3 | SCENE= }}
{{STRUCTURE_1fq3| PDB=1fq3 | SCENE= }}
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'''CRYSTAL STRUCTURE OF HUMAN GRANZYME B'''
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===CRYSTAL STRUCTURE OF HUMAN GRANZYME B===
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==Overview==
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Granzyme B is the prototypic member of the granzymes, a family of trypsin-like serine proteinases localized in the dense cytoplasmic granules of activated natural killer cells and cytotoxic T lymphocytes. Granzyme B directly triggers apoptosis in target cells by activating the caspase pathway, and has been implicated in the etiology of rheumatoid arthritis. Human granzyme B expressed in a baculovirus system has been crystallized without inhibitor and its structure has been determined to 3.1 A resolution, after considerably improving the diffraction power of the crystals by controlled humidity changes. The granzyme B structure reveals an overall fold similar to that found in cathepsin G and human chymase. The guanidinium group of Arg226, anchored at the back of the S1-specificity pocket, can form a salt bridge with the P1-Asp side chain of a bound peptide substrate. The architecture of the substrate binding site of granzyme B appears to be designed to accommodate and cleave hexapeptides such as the sequence Ile-Glu-Thr-Asp-/Ser-Gly present in the activation site of pro-caspase-3, a proven physiological substrate of granzyme B. These granzyme B crystals, with fully accessible active sites, are well suited for soaking with small synthetic inhibitors that might be used for a treatment of chronic inflammatory disorders.
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The line below this paragraph, {{ABSTRACT_PUBMED_11209755}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 11209755 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11209755}}
==About this Structure==
==About this Structure==
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[[Category: Rubin, H.]]
[[Category: Rubin, H.]]
[[Category: Chymotrypsin-like serine proteinase]]
[[Category: Chymotrypsin-like serine proteinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:37:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:45:56 2008''

Revision as of 00:45, 1 July 2008

Image:1fq3.png

Template:STRUCTURE 1fq3

CRYSTAL STRUCTURE OF HUMAN GRANZYME B

Template:ABSTRACT PUBMED 11209755

About this Structure

1FQ3 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of the caspase activator human granzyme B, a proteinase highly specific for an Asp-P1 residue., Estebanez-Perpina E, Fuentes-Prior P, Belorgey D, Braun M, Kiefersauer R, Maskos K, Huber R, Rubin H, Bode W, Biol Chem. 2000 Dec;381(12):1203-14. PMID:11209755

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