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| - | [[Image:1fuk.jpg|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1fuk| PDB=1fuk | SCENE= }} | | {{STRUCTURE_1fuk| PDB=1fuk | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A'''
| + | ===CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A=== |
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| - | ==Overview==
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| - | The eukaryotic translation initiation factor 4A (eIF4A) is a member of the DEA(D/H)-box RNA helicase family, a diverse group of proteins that couples an ATPase activity to RNA binding and unwinding. Previous work has provided the structure of the amino-terminal, ATP-binding domain of eIF4A. Extending those results, we have solved the structure of the carboxyl-terminal domain of eIF4A with data to 1.75 A resolution; it has a parallel alpha-beta topology that superimposes, with minor variations, on the structures and conserved motifs of the equivalent domain in other, distantly related helicases. Using data to 2.8 A resolution and molecular replacement with the refined model of the carboxyl-terminal domain, we have completed the structure of full-length eIF4A; it is a "dumbbell" structure consisting of two compact domains connected by an extended linker. By using the structures of other helicases as a template, compact structures can be modeled for eIF4A that suggest (i) helicase motif IV binds RNA; (ii) Arg-298, which is conserved in the DEA(D/H)-box RNA helicase family but is absent from many other helicases, also binds RNA; and (iii) motifs V and VI "link" the carboxyl-terminal domain to the amino-terminal domain through interactions with ATP and the DEA(D/H) motif, providing a mechanism for coupling ATP binding and hydrolysis with conformational changes that modulate RNA binding. | + | The line below this paragraph, {{ABSTRACT_PUBMED_11087862}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11087862 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11087862}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Helicase]] | | [[Category: Helicase]] |
| | [[Category: Initiation factor 4a]] | | [[Category: Initiation factor 4a]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:46:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 03:58:11 2008'' |
Revision as of 00:58, 1 July 2008
Template:STRUCTURE 1fuk
CRYSTAL STRUCTURE OF THE CARBOXY TERMINAL DOMAIN OF YEAST EIF4A
Template:ABSTRACT PUBMED 11087862
About this Structure
1FUK is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase., Caruthers JM, Johnson ER, McKay DB, Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13080-5. PMID:11087862
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