1fwi

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1fwi.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1fwi.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1fwi| PDB=1fwi | SCENE= }}
{{STRUCTURE_1fwi| PDB=1fwi | SCENE= }}
-
'''KLEBSIELLA AEROGENES UREASE, H134A VARIANT'''
+
===KLEBSIELLA AEROGENES UREASE, H134A VARIANT===
-
==Overview==
+
<!--
-
A mutant form of Klebsiella aerogenes urease possessing Ala instead of His at position 134 (H134A) is inactive and binds approximately half the normal complement of nickel (Park, I.-S., and Hausinger, R. P.(1993) Protein Sci. 2, 1034-1041). The crystal structure of the H134A protein was obtained at 2.0-A resolution, and it confirms that only Ni-1 of the two nickel ions found in the native enzyme is present. In contrast to the pseudotetrahedral geometry observed for Ni-1 in native urease (where it is liganded by His-246, His-272, one oxygen atom of carbamylated Lys-217, and a water molecule at partial occupancy), the mononickel metallocenter in the H134A protein was found to possess octahedral geometry and was coordinated by the above protein ligands plus three water molecules. The nickel site of H134A urease was probed by UV-visible, variable temperature magnetic circular dichroism, and x-ray absorption spectroscopies. The spectroscopic data are consistent with the presence of Ni(II) in octahedral geometry coordinated by two histidylimidazoles and additional oxygen and/or nitrogen donors. These data underscore the requirement of Ni-2 for formation of active urease and demonstrate the important role of Ni-2 in establishing the proper Ni-1 coordination geometry.
+
The line below this paragraph, {{ABSTRACT_PUBMED_8702515}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 8702515 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_8702515}}
==About this Structure==
==About this Structure==
Line 27: Line 31:
[[Category: Mutant]]
[[Category: Mutant]]
[[Category: Nickel metalloenzyme]]
[[Category: Nickel metalloenzyme]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 16:50:46 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:03:03 2008''

Revision as of 01:03, 1 July 2008

Image:1fwi.png

Template:STRUCTURE 1fwi

KLEBSIELLA AEROGENES UREASE, H134A VARIANT

Template:ABSTRACT PUBMED 8702515

About this Structure

1FWI is a Protein complex structure of sequences from Klebsiella aerogenes. Full crystallographic information is available from OCA.

Reference

Characterization of the mononickel metallocenter in H134A mutant urease., Park IS, Michel LO, Pearson MA, Jabri E, Karplus PA, Wang S, Dong J, Scott RA, Koehler BP, Johnson MK, Hausinger RP, J Biol Chem. 1996 Aug 2;271(31):18632-7. PMID:8702515

Page seeded by OCA on Tue Jul 1 04:03:03 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1fwi"
Personal tools