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1g15

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{{STRUCTURE_1g15| PDB=1g15 | SCENE= }}
{{STRUCTURE_1g15| PDB=1g15 | SCENE= }}
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'''CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE'''
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===CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE===
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==Overview==
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Ribonuclease H (RNase H) selectively degrades the RNA strand of RNA.DNA hybrids in a divalent cation-dependent manner. Previous structural studies revealed a single Mg(2+) ion-binding site in Escherichia coli RNase HI. In the crystal structure of the related RNase H domain of human immunodeficiency virus reverse transcriptase, however, two Mn(2+) ions were observed suggesting a different mode of metal binding. E. coli RNase HI shows catalytic activity in the presence of Mg(2+) or Mn(2+) ions, but these two metals show strikingly different optimal concentrations. Mg(2+) ions are required in millimolar concentrations, but Mn(2+) ions are only required in micromolar quantities. Based upon the metal dependence of E. coli RNase HI activity, we proposed an activation/attenuation model in which one metal is required for catalysis, and binding of a second metal is inhibitory. We have now solved the co-crystal structure of E. coli RNase HI with Mn(2+) ions at 1.9-A resolution. Two octahedrally coordinated Mn(2+) ions are seen to bind to the enzyme-active site. Residues Asp-10, Glu-48, and Asp-70 make direct (inner sphere) coordination contacts to the first (activating) metal, whereas residues Asp-10 and Asp-134 make direct contacts to the second (attenuating) metal. This structure is consistent with biochemical evidence suggesting that two metal ions may bind RNase H but liganding a second ion inhibits RNase H activity.
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{{ABSTRACT_PUBMED_11083878}}
==About this Structure==
==About this Structure==
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[[Category: Ribonuclease h]]
[[Category: Ribonuclease h]]
[[Category: Rnase h]]
[[Category: Rnase h]]
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Revision as of 01:15, 1 July 2008

Image:1g15.png

Template:STRUCTURE 1g15

CO-CRYSTAL OF E. COLI RNASE HI WITH TWO MN2+ IONS BOUND IN THE THE ACTIVE SITE

Template:ABSTRACT PUBMED 11083878

About this Structure

1G15 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Co-crystal of Escherichia coli RNase HI with Mn2+ ions reveals two divalent metals bound in the active site., Goedken ER, Marqusee S, J Biol Chem. 2001 Mar 9;276(10):7266-71. Epub 2000 Nov 16. PMID:11083878

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