1gsj
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1gsj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gsj, resolution 1.85Å" /> '''SELENOMETHIONINE SU...)
Next diff →
Revision as of 17:45, 29 October 2007
|
SELENOMETHIONINE SUBSTITUTED N-ACETYL-L-GLUTAMATE KINASE FROM ESCHERICHIA COLI COMPLEXED WITH ITS SUBSTRATE N-ACETYL-L-GLUTAMATE AND ITS SUBSTRATE ANALOG AMPPNP
Overview
N-Acetyl-L-glutamate kinase (NAGK), a member of the amino acid kinase, family, catalyzes the second and frequently controlling step of arginine, synthesis. The Escherichia coli NAGK crystal structure to 1.5 A resolution, reveals a 258-residue subunit homodimer nucleated by a central 16-stranded, molecular open beta sheet sandwiched between alpha helices. In each, subunit, AMPPNP, as an alphabetagamma-phosphate-Mg2+ complex, binds along, the sheet C edge, and N-acetyl-L-glutamate binds near the dyadic axis with, its gamma-COO- aligned at short distance from the gamma-phosphoryl, indicating associative phosphoryl transfer assisted by: (1) Mg2+, complexation; (2) the positive charges on Lys8, Lys217, and on two helix, dipoles; and (3) by hydrogen bonding with the y-phosphate. The structural, ... [(full description)]
About this Structure
1GSJ is a [Single protein] structure of sequence from [Escherichia coli] with MG, NLG and ANP as [ligands]. Active as [[1]], with EC number [2.7.2.8]. Full crystallographic information is available from [OCA].
Reference
Structure of acetylglutamate kinase, a key enzyme for arginine biosynthesis and a prototype for the amino acid kinase enzyme family, during catalysis., Ramon-Maiques S, Marina A, Gil-Ortiz F, Fita I, Rubio V, Structure. 2002 Mar;10(3):329-42. PMID:12005432
Page seeded by OCA on Mon Oct 29 19:50:26 2007
