1g4g

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{{STRUCTURE_1g4g| PDB=1g4g | SCENE= }}
{{STRUCTURE_1g4g| PDB=1g4g | SCENE= }}
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'''NMR STRUCTURE OF THE FIFTH DOMAIN OF HUMAN BETA2-GLYCOPROTEIN I'''
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===NMR STRUCTURE OF THE FIFTH DOMAIN OF HUMAN BETA2-GLYCOPROTEIN I===
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==Overview==
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To understand the mechanism of the interaction between human beta(2)-glycoprotein I (beta(2)-GPI) and negatively charged phospholipids, we determined the three-dimensional solution structure of the fifth domain of beta(2)-GPI by heteronuclear multidimensional NMR. The results showed that the molecule is composed of well-defined four anti-parallel beta-strands and two short alpha-helices, as well as a long highly flexible loop. Backbone dynamic analysis demonstrated significant mobility of the flexible loop on a subnanosecond time scale. Structural modeling of the nicked fifth domain, in which the Lys317-Thr318 peptide bond was specifically cleaved, revealed the importance of this long C-terminal loop for the interaction between beta(2)-GPI and negatively charged phospholipids. A titration experiment with the anionic surfactant SDS showed that this highly mobile loop, as well as the short beta-hairpin between betaC and betaD strands, which is rich in positively charged residues, specifically interact with the surfactant. The mobile loop, together with the surrounding positively charged residues, probably construct the binding site for negatively charged phospholipids such as cardiolipin.
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{{ABSTRACT_PUBMED_11124037}}
==About this Structure==
==About this Structure==
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1G4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4G OCA].
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1G4G is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1G4G OCA].
==Reference==
==Reference==
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[[Category: Short consensus repeat]]
[[Category: Short consensus repeat]]
[[Category: Sushi-domain]]
[[Category: Sushi-domain]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:07:41 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:23:56 2008''

Revision as of 01:23, 1 July 2008

Image:1g4g.png

Template:STRUCTURE 1g4g

NMR STRUCTURE OF THE FIFTH DOMAIN OF HUMAN BETA2-GLYCOPROTEIN I

Template:ABSTRACT PUBMED 11124037

About this Structure

1G4G is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

Identification of the phospholipid-binding site of human beta(2)-glycoprotein I domain V by heteronuclear magnetic resonance., Hoshino M, Hagihara Y, Nishii I, Yamazaki T, Kato H, Goto Y, J Mol Biol. 2000 Dec 15;304(5):927-39. PMID:11124037

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