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| | {{STRUCTURE_1g4i| PDB=1g4i | SCENE= }} | | {{STRUCTURE_1g4i| PDB=1g4i | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE BOVINE PANCREATIC PHOSPHOLIPASE A2 AT 0.97A'''
| + | ===CRYSTAL STRUCTURE OF THE BOVINE PANCREATIC PHOSPHOLIPASE A2 AT 0.97A=== |
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| - | ==Overview==
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| - | Using synchrotron radiation and a CCD camera, X-ray data have been collected from wild-type bovine pancreatic phospholipase A(2) at 100 K to 0.97 A resolution allowing full anisotropic refinement. The final model has a conventional R factor of 9.44% for all reflections, with a mean standard uncertainty for the positional parameters of 0.031 A as calculated from inversion of the full positional least-squares matrix. At 0.97 A resolution, bovine pancreatic phospholipase A(2) reveals for the first time that its rigid scaffolding does not preclude flexibility, which probably plays an important role in the catalytic process. Functionally important regions (the interfacial binding site and calcium-binding loop) are located at the molecular surface, where conformational variability is more pronounced. A cluster of 2-methyl-2,4-pentanediol molecules is present at the entrance of the hydrophobic channel that leads to the catalytic site and mimics the fatty-acid chains of a substrate analogue. Bovine pancreatic phospholipase A(2) at atomic resolution is compared with previous crystallographic structures and with models derived from nuclear magnetic resonance studies. Given the high structural similarity among extracellular phospholipases A(2) observed so far at lower resolution, the results arising from this structural analysis are expected to be of general validity for this class of enzymes.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_11264580}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 11264580 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_11264580}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Wilson, K S.]] | | [[Category: Wilson, K S.]] |
| | [[Category: Lipid degradation]] | | [[Category: Lipid degradation]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:07:43 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:24:15 2008'' |
Revision as of 01:24, 1 July 2008
Template:STRUCTURE 1g4i
CRYSTAL STRUCTURE OF THE BOVINE PANCREATIC PHOSPHOLIPASE A2 AT 0.97A
Template:ABSTRACT PUBMED 11264580
About this Structure
1G4I is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
X-ray structure of bovine pancreatic phospholipase A2 at atomic resolution., Steiner RA, Rozeboom HJ, de Vries A, Kalk KH, Murshudov GN, Wilson KS, Dijkstra BW, Acta Crystallogr D Biol Crystallogr. 2001 Apr;57(Pt 4):516-26. PMID:11264580
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