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1g59

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{{STRUCTURE_1g59| PDB=1g59 | SCENE= }}
{{STRUCTURE_1g59| PDB=1g59 | SCENE= }}
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'''GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).'''
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===GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).===
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==Overview==
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Glutamyl-tRNA synthetases (GluRSs) are divided into two distinct types, with regard to the presence or absence of glutaminyl-tRNA synthetase (GlnRS) in the genetic translation systems. In the original 19-synthetase systems lacking GlnRS, the 'non-discriminating' GluRS glutamylates both tRNAGlu and tRNAGln. In contrast, in the evolved 20-synthetase systems with GlnRS, the 'discriminating' GluRS aminoacylates only tRNAGlu. Here we report the 2.4 A resolution crystal structure of a 'discriminating' GluRS.tRNAGlu complex from Thermus thermophilus. The GluRS recognizes the tRNAGlu anticodon bases via two alpha-helical domains, maintaining the base stacking. We show that the discrimination between the Glu and Gln anticodons (34YUC36 and 34YUG36, respectively) is achieved by a single arginine residue (Arg 358). The mutation of Arg 358 to Gln resulted in a GluRS that does not discriminate between the Glu and Gln anticodons. This change mimics the reverse course of GluRS evolution from anticodon 'non-dicsriminating' to 'discriminating'.
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(as it appears on PubMed at http://www.pubmed.gov), where 11224561 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11224561}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transfer rna]]
[[Category: Transfer rna]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:09:04 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:26:32 2008''

Revision as of 01:26, 1 July 2008

Image:1g59.png

Template:STRUCTURE 1g59

GLUTAMYL-TRNA SYNTHETASE COMPLEXED WITH TRNA(GLU).

Template:ABSTRACT PUBMED 11224561

About this Structure

1G59 is a Single protein structure of sequence from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase., Sekine S, Nureki O, Shimada A, Vassylyev DG, Yokoyama S, Nat Struct Biol. 2001 Mar;8(3):203-6. PMID:11224561

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