1g5q

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{{STRUCTURE_1g5q| PDB=1g5q | SCENE= }}
{{STRUCTURE_1g5q| PDB=1g5q | SCENE= }}
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'''EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC'''
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===EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC===
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==Overview==
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Epidermin from Staphylococcus epidermidis Tu3298 is an antimicrobial peptide of the lantibiotic family that contains, amongst other unusual amino acids, S:-[(Z:)- 2-aminovinyl]-D-cysteine. This residue is introduced by post-translational modification of the ribosomally synthesized precursor EpiA. Modification starts with the oxidative decarboxylation of its C-terminal cysteine by the flavoprotein EpiD generating a reactive (Z:)-enethiol intermediate. We have determined the crystal structures of EpiD and EpiD H67N in complex with the substrate pentapeptide DSYTC at 2.5 A resolution. Rossmann-type monomers build up a dodecamer of 23 point symmetry with trimers disposed at the vertices of a tetrahedron. Oligomer formation is essential for binding of flavin mononucleotide and substrate, which is buried by an otherwise disordered substrate recognition clamp. A pocket for the tyrosine residue of the substrate peptide is formed by an induced fit mechanism. The substrate contacts flavin mononucleotide only via Cys-Sgamma, suggesting its oxidation as the initial step. A thioaldehyde intermediate could undergo spontaneous decarboxylation. The unusual substrate recognition mode and the type of chemical reaction performed provide insight into a novel family of flavoproteins.
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(as it appears on PubMed at http://www.pubmed.gov), where 11101502 is the PubMed ID number.
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{{ABSTRACT_PUBMED_11101502}}
==About this Structure==
==About this Structure==
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[[Category: Beta protein]]
[[Category: Beta protein]]
[[Category: Rossman like fold]]
[[Category: Rossman like fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 17:10:12 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 1 04:29:08 2008''

Revision as of 01:29, 1 July 2008

Image:1g5q.png

Template:STRUCTURE 1g5q

EPID H67N COMPLEXED WITH SUBSTRATE PEPTIDE DSYTC

Template:ABSTRACT PUBMED 11101502

About this Structure

1G5Q is a Protein complex structure of sequences from Staphylococcus epidermidis. Full crystallographic information is available from OCA.

Reference

Crystal structure of the peptidyl-cysteine decarboxylase EpiD complexed with a pentapeptide substrate., Blaesse M, Kupke T, Huber R, Steinbacher S, EMBO J. 2000 Dec 1;19(23):6299-310. PMID:11101502

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